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oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21
Joint Authors
Lambert, Marius
Pfeiler, Susanne
Kopp, Reinhard
Meimarakis, Georgios
Sicic, Zeljka
Lackermair, Korbinian
David, Robert
Weis, Michael
Summo, Claudia
Beiras-Fernandez, A.
Nickel, Thomas
Kaczmarek, Ingo
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
Dendritic cells (DCs) and oxLDL play an important role in the atherosclerotic process with DCs accumulating in the plaques during plaque progression.
Our aim was to investigate the role of oxLDL in the modulation of the DC homing-receptor CCR7 and endothelial-ligand CCL21.
Methods and Results.
The expression of the DC homing-receptor CCR7 and its endothelial-ligand CCL21 was examined on atherosclerotic carotic plaques of 47 patients via qRT-PCR and immunofluorescence.
In vitro, we studied the expression of CCR7 on DCs and CCL21 on human microvascular endothelial cells (HMECs) in response to oxLDL.
CCL21- and CCR7-mRNA levels were significantly downregulated in atherosclerotic plaques versus non-atherosclerotic controls [90% for CCL21 and 81% for CCR7 (P<0.01)].
In vitro, oxLDL reduced CCR7 mRNA levels on DCs by 30% and protein levels by 46%.
Furthermore, mRNA expression of CCL21 was significantly reduced by 50% (P<0.05) and protein expression by 24% in HMECs by oxLDL (P<0.05).
Conclusions.
The accumulation of DCs in atherosclerotic plaques appears to be related to a downregulation of chemokines and their ligands, which are known to regulate DC migration.
oxLDL induces an in vitro downregulation of CCR7 and CCL21, which may play a role in the reduction of DC migration from the plaques.
American Psychological Association (APA)
Nickel, Thomas& Pfeiler, Susanne& Summo, Claudia& Kopp, Reinhard& Meimarakis, Georgios& Sicic, Zeljka…[et al.]. 2012. oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1029391
Modern Language Association (MLA)
Nickel, Thomas…[et al.]. oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21. Mediators of Inflammation No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1029391
American Medical Association (AMA)
Nickel, Thomas& Pfeiler, Susanne& Summo, Claudia& Kopp, Reinhard& Meimarakis, Georgios& Sicic, Zeljka…[et al.]. oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1029391
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029391