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Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser
Joint Authors
Leenen, L. P. H.
Groeneveld, Kathelijne Maaike
Heeres, Marjolein
Huisman, Albert
Koenderman, Leo
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Introduction.
Flow cytometry markers have been proposed as useful predictors for the occurrence of posttraumatic inflammatory complications.
However, currently the need for a dedicated laboratory and the labour-intensive analytical procedures make these markers less suitable for clinical practice.
We tested an approach to overcome these limitations.
Material and Methods.
Neutrophils of healthy donors were incubated with antibodies commonly used in trauma research: CD11b (MAC-1), L-selectin (CD62L), FcγRIII (CD16), and FcγRII (CD32) in active form (MoPhab A27).
Flow cytometric analysis was performed both on a FACSCalibur, a standard flow cytometer, and on a Cell-Dyn Sapphire, a routine haematology analyser.
Results.
There was a high level of agreement between the two types of analysers, with 41% for FcγRIII, 80% for L-selectin, 98% for CD11b, and even a 100% agreement for active FcγRII.
Moreover, analysis on the routine haematology analyser was possible in less than a quarter of the time in comparison to the flow cytometer.
Conclusion.
Analysis of neutrophil phenotype on the Cell-Dyn Sapphire leads to the same conclusion compared to a standard flow cytometer.
The markedly reduced time necessary for analysis and reduced labour intensity constitutes a step forward in implementation of this type of analysis in clinical diagnostics in trauma research.
American Psychological Association (APA)
Groeneveld, Kathelijne Maaike& Heeres, Marjolein& Leenen, L. P. H.& Huisman, Albert& Koenderman, Leo. 2012. Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029410
Modern Language Association (MLA)
Groeneveld, Kathelijne Maaike…[et al.]. Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser. Mediators of Inflammation No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-1029410
American Medical Association (AMA)
Groeneveld, Kathelijne Maaike& Heeres, Marjolein& Leenen, L. P. H.& Huisman, Albert& Koenderman, Leo. Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029410