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Molecular Mechanism of Macrophage Activation by Red Ginseng Acidic Polysaccharide from Korean Red Ginseng
Joint Authors
Yang, Woo Seok
Kim, Ji Hye
Lee, Jaehwi
Byeon, Se Eun
Kwak, Yi-Seong
Kim, Sun Young
Choung, Eui Su
Cho, Jae Youl
Rhee, Man Hee
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng, displays immunostimulatory and antitumor activities.
Even though numerous studies have been reported, the mechanism as to how RGAP is able to stimulate the immune response is not clear.
In this study, we aimed to explore the mechanism of molecular activation of RGAP in macrophages.
RGAP treatment strongly induced NO production in RAW264.7 cells without altering morphological changes, although the activity was not strong compared to LPS-induced dendritic-like morphology in RAW264.7 cells.
RGAP-induced NO production was accompanied with enhanced mRNA levels of iNOS and increases in nuclear transcription factors such as NF-κB, AP-1, STAT-1, ATF-2, and CREB.
According to pharmacological evaluation with specific enzyme inhibitors, Western blot analysis of intracellular signaling proteins and inhibitory pattern using blocking antibodies, ERK, and JNK were found to be the most important signaling enzymes compared to LPS signaling cascade.
Further, TLR2 seems to be a target surface receptor of RGAP.
Lastly, macrophages isolated from RGS2 knockout mice or wortmannin exposure strongly upregulated RGAP-treated NO production.
Therefore, our results suggest that RGAP can activate macrophage function through activation of transcription factors such as NF-κB and AP-1 and their upstream signaling enzymes such as ERK and JNK.
American Psychological Association (APA)
Byeon, Se Eun& Lee, Jaehwi& Kim, Ji Hye& Yang, Woo Seok& Kwak, Yi-Seong& Kim, Sun Young…[et al.]. 2012. Molecular Mechanism of Macrophage Activation by Red Ginseng Acidic Polysaccharide from Korean Red Ginseng. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029435
Modern Language Association (MLA)
Byeon, Se Eun…[et al.]. Molecular Mechanism of Macrophage Activation by Red Ginseng Acidic Polysaccharide from Korean Red Ginseng. Mediators of Inflammation No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1029435
American Medical Association (AMA)
Byeon, Se Eun& Lee, Jaehwi& Kim, Ji Hye& Yang, Woo Seok& Kwak, Yi-Seong& Kim, Sun Young…[et al.]. Molecular Mechanism of Macrophage Activation by Red Ginseng Acidic Polysaccharide from Korean Red Ginseng. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029435
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029435