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Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions
Joint Authors
Wang, Jinmei
You, X. Joann
Gu, Ping
Klassen, Henry
Yang, Jing
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-23
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Neural progenitor cells (NPCs) of feline origin (cNPCs) have demonstrated utility in transplantation experiments, yet are difficult to grow in culture beyond the 1 month time frame.
Here we use an enriched, serum-free base medium (Ultraculture) and report the successful long-term propagation of these cells.
Primary cultures were derived from fetal brain tissue and passaged in DMEM/F12-based or Ultraculture-based proliferation media, both in the presence of EGF + bFGF.
Cells in standard DMEM/F12-based medium ceased to proliferate by 1-month, whereas the cells in the Ultraculture-based medium continued to grow for at least 5 months (end of study) with no evidence of senescence.
The Ultraculture-based cultures expressed lower levels of progenitor and lineage-associated markers under proliferation conditions but retained multipotency as evidenced by the ability to differentiate into neurons and glia following growth factor removal in the presence of FBS.
Importantly, later passage cNPCs did not develop chromosomal aberrations.
American Psychological Association (APA)
Yang, Jing& Wang, Jinmei& Gu, Ping& You, X. Joann& Klassen, Henry. 2012. Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions. Stem Cells International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1029997
Modern Language Association (MLA)
Yang, Jing…[et al.]. Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions. Stem Cells International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1029997
American Medical Association (AMA)
Yang, Jing& Wang, Jinmei& Gu, Ping& You, X. Joann& Klassen, Henry. Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1029997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029997