Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study
Joint Authors
Marmotti, A.
Mattia, S.
Bruzzone, M.
Buttiglieri, S.
Risso, A.
Blonna, D.
Rossi, R.
Zanini, C.
Ercole, E.
Defabiani, E.
Tarella, C.
Peretti, G. M.
Castoldi, Filippo
Bonasia, Davide Edoardo
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-07
Country of Publication
Egypt
No. of Pages
13
Abstract EN
A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering.
The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs.
We obtained, at passage 1 (P1), a mean value of 4,2×106 cells (SD 0,4) from each UC.
At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II.
Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated.
Telomere length was similar to that of bone-marrow (BM) MSCs from young donors.
The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.
American Psychological Association (APA)
Marmotti, A.& Mattia, S.& Bruzzone, M.& Buttiglieri, S.& Risso, A.& Bonasia, Davide Edoardo…[et al.]. 2012. Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study. Stem Cells International،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1030009
Modern Language Association (MLA)
Marmotti, A.…[et al.]. Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study. Stem Cells International No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1030009
American Medical Association (AMA)
Marmotti, A.& Mattia, S.& Bruzzone, M.& Buttiglieri, S.& Risso, A.& Bonasia, Davide Edoardo…[et al.]. Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1030009
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030009
