Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH
Joint Authors
Montioli, Riccardo
Dindo, Mirco
Oppici, Elisa
Voltattorni, Carla Borri
Cellini, Barbara
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Human Dopa decarboxylase (hDDC), a pyridoxal 5′-phosphate (PLP) enzyme, displays maxima at 420 and 335 nm and emits fluorescence at 384 and 504 nm upon excitation at 335 nm and at 504 nm when excited at 420 nm.
Absorbance and fluorescence titrations of hDDC-bound coenzyme identify a single pKspec of ~7.2.
This pKspec could not represent the ionization of a functional group on the Schiff base but that of an enzymic residue governing the equilibrium between the low- and the high-pH forms of the internal aldimine.
During the reaction of hDDC with L-Dopa, monitored by stopped-flow spectrophotometry, a 420 nm band attributed to the 4′-N-protonated external aldimine first appears, and its decrease parallels the emergence of a 390 nm peak, assigned to the 4′-N-unprotonated external aldimine.
The pH profile of the spectral change at 390 nm displays a pK of 6.4, a value similar to that (~6.3) observed in both kcat and kcat/Km profiles.
This suggests that this pK represents the ESH+ → ES catalytic step.
The assignment of the pKs of 7.9 and 8.3 observed on the basic side of kcat and the PLP binding affinity profiles, respectively, is also analyzed and discussed.
American Psychological Association (APA)
Montioli, Riccardo& Cellini, Barbara& Dindo, Mirco& Oppici, Elisa& Voltattorni, Carla Borri. 2013. Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030145
Modern Language Association (MLA)
Montioli, Riccardo…[et al.]. Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030145
American Medical Association (AMA)
Montioli, Riccardo& Cellini, Barbara& Dindo, Mirco& Oppici, Elisa& Voltattorni, Carla Borri. Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030145
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030145