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Mutations in the embB Gene and Their Association with Ethambutol Resistance in Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates from Poland
Joint Authors
Augustynowicz-Kopeć, Ewa
Jagielski, Tomasz
Bakuła, Zofia
Napiórkowska, Agnieszka
Zwolska, Zofia
Bielecki, Jacek
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Ethambutol (EMB) continues to be used as part of a standard drug regimen for the treatment of tuberculosis (TB).
Mutations in the embB gene and those within its conserved EMB resistance determining region (ERDR) in particular have repeatedly been associated with resistance to EMB in Mycobacterium tuberculosis.
The aim of this study was to examine the mutational “hot spots” in the embB gene, including the ERDR, among multidrug-resistant (MDR) M.
tuberculosis clinical isolates and to find a possible association between embB mutations and resistance to EMB.
An 863-bp fragment of the embB gene was sequenced in 17 EMB-resistant and 33 EMB-susceptible MDR-TB isolates.
In total, eight embB mutation types were detected in 6 distinct codons of 27 (54%) M.
tuberculosis isolates.
Mutations in codon 306 were most common, found in both EMB-resistant (9) and EMB-susceptible (11) isolates.
Only mutations in codons 406 and 507 were found exclusively in four and one EMB-resistant isolates, respectively.
Sequence analysis of the ERDR in the embB gene is not sufficient for rapid detection of EMB resistance, and the codon 306 mutations are not good predictive markers of resistance to EMB.
American Psychological Association (APA)
Bakuła, Zofia& Napiórkowska, Agnieszka& Bielecki, Jacek& Augustynowicz-Kopeć, Ewa& Zwolska, Zofia& Jagielski, Tomasz. 2013. Mutations in the embB Gene and Their Association with Ethambutol Resistance in Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates from Poland. BioMed Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1030148
Modern Language Association (MLA)
Bakuła, Zofia…[et al.]. Mutations in the embB Gene and Their Association with Ethambutol Resistance in Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates from Poland. BioMed Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1030148
American Medical Association (AMA)
Bakuła, Zofia& Napiórkowska, Agnieszka& Bielecki, Jacek& Augustynowicz-Kopeć, Ewa& Zwolska, Zofia& Jagielski, Tomasz. Mutations in the embB Gene and Their Association with Ethambutol Resistance in Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates from Poland. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1030148
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030148