In Vitro Ovicidal and Cestocidal Effects of Toxins from Bacillus thuringiensis on the Canine and Human Parasite Dipylidium caninum
Joint Authors
Peña, Guadalupe
Aguilar Jiménez, Fortino Agustín
Hallal-Calleros, Claudia
Morales-Montor, Jorge
Flores-Pérez, Fernando Iván
Hernández-Velázquez, Víctor M.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Bacillus thuringiensis is a gram-positive soil-dwelling bacterium that is commonly used as a biological pesticide.
This bacterium may also be used for biological control of helminth parasites in domestic animals.
In this study, we evaluated the possible ovicidal and cestocidal effects of a total protein extract of B.
thuringiensis native strains on the zoonotic cestode parasite of dogs, Dipylidium caninum (D.
caninum).
Dose and time response curves were determined by coincubating B.
thuringiensis proteins at concentration ranging from 100 to 1000 μg/mL along with 4000 egg capsules of D.
caninum.
Egg viability was evaluated using the trypan blue exclusion test.
The lethal concentration of toxins on eggs was 600 μg/ml, and the best incubation time to produce this effect was 3 h.
In the adult stage, the motility and the thickness of the tegument were used as indicators of damage.
The motility was inhibited by 100% after 8 hours of culture compared to the control group, while the thickness of the cestode was reduced by 34%.
Conclusively, proteins of the strain GP526 of B.
thuringiensis directly act upon D.
caninum showing ovicidal and cestocidal effects.
Thus, B.
thuringiensis is proposed as a potential biological control agent against this zoonosis.
American Psychological Association (APA)
Peña, Guadalupe& Aguilar Jiménez, Fortino Agustín& Hallal-Calleros, Claudia& Morales-Montor, Jorge& Hernández-Velázquez, Víctor M.& Flores-Pérez, Fernando Iván. 2012. In Vitro Ovicidal and Cestocidal Effects of Toxins from Bacillus thuringiensis on the Canine and Human Parasite Dipylidium caninum. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030155
Modern Language Association (MLA)
Peña, Guadalupe…[et al.]. In Vitro Ovicidal and Cestocidal Effects of Toxins from Bacillus thuringiensis on the Canine and Human Parasite Dipylidium caninum. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030155
American Medical Association (AMA)
Peña, Guadalupe& Aguilar Jiménez, Fortino Agustín& Hallal-Calleros, Claudia& Morales-Montor, Jorge& Hernández-Velázquez, Víctor M.& Flores-Pérez, Fernando Iván. In Vitro Ovicidal and Cestocidal Effects of Toxins from Bacillus thuringiensis on the Canine and Human Parasite Dipylidium caninum. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030155
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030155