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Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem
Joint Authors
Amara, Amro A.
Haroun, Bakry M.
Serour, Ehab A.
Hassan, Mohamed A.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-11
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Novel keratin-degrading bacteria were isolated from sand soil samples collected from Minia Governorate, Egypt.
In this study, the isolates were identified as Bacillus amyloliquefaciens MA20 and Bacillus subtilis MA21 based on morphological and biochemical characteristics as well as 16S rRNA gene sequencing.
B.
amyloliquefaciens MA20 and B.
subtilis MA21 produced alkaline keratinolytic serine protease when cultivated in mineral medium containing 1% of wool straight off sheep as sole carbon and nitrogen source.
The two strains were observed to degrade wool completely to powder at pH 7 and 37°C within 5 days.
Under these conditions the maximum activity of proteases produced by B.
amyloliquefaciens MA20 and B.
subtilis MA21 was 922 and 814 U/ml, respectively.
The proteases exhibited optimum temperature and pH at 60°C and 9, respectively.
However, the keratinolytic proteases were stable in broad range of temperature and pH values towards casein Hammerstein.
Furthermore the protease inhibitor studies indicated that the produced proteases belong to serine protease because of their sensitivity to PMSF while they were inhibited partially in presence of EDTA.
The two proteases are stable in most of the used organic solvents and enhanced by metals suggesting their potential use in biotechnological applications such as wool industry.
American Psychological Association (APA)
Hassan, Mohamed A.& Haroun, Bakry M.& Amara, Amro A.& Serour, Ehab A.. 2013. Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem. BioMed Research International،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1030156
Modern Language Association (MLA)
Hassan, Mohamed A.…[et al.]. Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem. BioMed Research International No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-1030156
American Medical Association (AMA)
Hassan, Mohamed A.& Haroun, Bakry M.& Amara, Amro A.& Serour, Ehab A.. Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1030156
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030156