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The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility
Joint Authors
Orchel, Arkadiusz
Jelonek, Katarzyna
Kasperczyk, Janusz
Marcinkowski, Andrzej
Pamula, Elzbieta
Orchel, Joanna
Bielecki, Ireneusz
Kulczycka, Anna
Dobrzynski, P.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Because of the wide use of biodegradable materials in tissue engineering, it is necessary to obtain biocompatible polymers with different mechanical and physical properties as well as degradation ratio.
Novel co- and terpolymers of various composition and chain microstructure have been developed and applied for cell culture.
The aim of this study was to evaluate the adhesion and proliferation of human chondrocytes to four biodegradable copolymers: lactide-coglycolide, lactide-co-ε-caprolactone, lactide-co-trimethylene carbonate, glycolide-co-ε-caprolactone, and one terpolymer glycolide-colactide-co-ε-caprolactone synthesized with the use of zirconium acetylacetonate as a nontoxic initiator.
Chain microstructure of the copolymers was analyzed by means of 1H and 13C NMR spectroscopy and surface properties by AFM technique.
Cell adhesion and proliferation were determined by CyQUANT Cell Proliferation Assay Kit.
After 4 h the chondrocyte adhesion on the surface of studied materials was comparable to standard TCPS.
Cell proliferation occurred on all the substrates; however, among the studied polymers poly(L-lactide-coglycolide) 85 : 15 that characterized the most blocky structure best supported cell growth.
Chondrocytes retained the cell membrane integrity evaluated by the LDH release assay.
As can be summarized from the results of the study, all the studied polymers are well tolerated by the cells that make them appropriate for human chondrocytes growth.
American Psychological Association (APA)
Orchel, Arkadiusz& Jelonek, Katarzyna& Kasperczyk, Janusz& Dobrzynski, P.& Marcinkowski, Andrzej& Pamula, Elzbieta…[et al.]. 2013. The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030158
Modern Language Association (MLA)
Orchel, Arkadiusz…[et al.]. The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030158
American Medical Association (AMA)
Orchel, Arkadiusz& Jelonek, Katarzyna& Kasperczyk, Janusz& Dobrzynski, P.& Marcinkowski, Andrzej& Pamula, Elzbieta…[et al.]. The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030158
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030158