MDR Gene Expression Analysis of Six Drug-Resistant Ovarian Cancer Cell Lines
Joint Authors
Januchowski, Radosław
Zabel, Maciej
Wojtowicz, Karolina
Sujka-Kordowska, Patrycja
Andrzejewska, Małgorzata
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ovarian cancer is the leading cause of death among gynaecological malignancies.
Multiple drug resistance makes cancer cells insensitive to chemotherapy.
In this study, we developed sixprimary ovarian cancer cell lines (W1MR, W1CR, W1DR, W1VR, W1TR, and W1PR) resistant to drugs such as methotrexate, cisplatin, doxorubicin, vincristine, topotecan, and paclitaxel.
A chemosensitivity assay MTT test was performed to assess drug cross-resistance.
Quantitative real-time polymerase chain reaction and Western blot were also performed to determine mRNA and protein expression of genes involved in chemoresistance.
We observed high cross-resistance to doxorubicin, vincristine, and paclitaxel in the cell lines resistant to these agents.
We also found a significant correlation between resistance to these drugs and increased expression of P-gp.
Two different mechanisms of topotecan resistance were observed in the W1TR and W1PR cell lines.
We did not observe any correlation between MRP2 transcriptand protein levels.
Cell lines resistant to agents used in ovarian cancer treatment remained sensitive to methotrexate.
The main mechanisms of drug resistance were due to P-gp expression in the doxorubicin, vincristine, and paclitaxel resistant cell lines and BCRP expression in the topotecan resistant cell line.
American Psychological Association (APA)
Januchowski, Radosław& Wojtowicz, Karolina& Sujka-Kordowska, Patrycja& Andrzejewska, Małgorzata& Zabel, Maciej. 2012. MDR Gene Expression Analysis of Six Drug-Resistant Ovarian Cancer Cell Lines. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030219
Modern Language Association (MLA)
Januchowski, Radosław…[et al.]. MDR Gene Expression Analysis of Six Drug-Resistant Ovarian Cancer Cell Lines. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030219
American Medical Association (AMA)
Januchowski, Radosław& Wojtowicz, Karolina& Sujka-Kordowska, Patrycja& Andrzejewska, Małgorzata& Zabel, Maciej. MDR Gene Expression Analysis of Six Drug-Resistant Ovarian Cancer Cell Lines. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030219