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Viability Reduction and Rac1 Gene Downregulation of Heterogeneous Ex-Vivo Glioma Acute Slice Infected by the Oncolytic Newcastle Disease Virus Strain V4UPM
Joint Authors
Aini, I.
Abdullah, Jafri Malin
Shamsuddin, Hilda Shazana
Ibrahim, Rohaya
Jaafar, Hasnan
Ali, Abdul Manaf
Mustafa, Zulkifli
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Oncolytic viruses have been extensively evaluated for anticancer therapy because this virus preferentially infects cancer cells without interfering with normal cells.
Newcastle Disease Virus (NDV) is an avian virus and one of the intensively studied oncolytic viruses affecting many types of cancer including glioma.
Nevertheless, the capability of NDV infection on heterogeneous glioma tissue in a cerebrospinal fluid atmosphere has never been reported.
Recently, Rac1 is reported to be required for efficient NDV replication in human cancer cells and established a link between tumourigenesis and sensitivity to NDV.
Rac1 is a member of the Rho GTPases involved in the regulation of the cell migration and cell-cycle progression.
Rac1 knockdown leads to significant inhibition of viral replication.
In this work, we demonstrated that NDV treatment led to significant reduction of tumour tissue viability of freshly isolated heterogeneous human brain tumour slice, known as an ex vivo glioma acute slice (EGAS).
Analysis of gene expression indicated that reduced tissue viability was associated with downregulation of Rac1.
However, the viability reduction was not persistent.
We conclude that NDV treatment induced EGAS viability suppression, but subsequent downregulation of Rac1 gene may reduce the NDV replication and lead to regrowth of EGAS tissue.
American Psychological Association (APA)
Mustafa, Zulkifli& Shamsuddin, Hilda Shazana& Aini, I.& Ibrahim, Rohaya& Jaafar, Hasnan& Ali, Abdul Manaf…[et al.]. 2013. Viability Reduction and Rac1 Gene Downregulation of Heterogeneous Ex-Vivo Glioma Acute Slice Infected by the Oncolytic Newcastle Disease Virus Strain V4UPM. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030226
Modern Language Association (MLA)
Mustafa, Zulkifli…[et al.]. Viability Reduction and Rac1 Gene Downregulation of Heterogeneous Ex-Vivo Glioma Acute Slice Infected by the Oncolytic Newcastle Disease Virus Strain V4UPM. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030226
American Medical Association (AMA)
Mustafa, Zulkifli& Shamsuddin, Hilda Shazana& Aini, I.& Ibrahim, Rohaya& Jaafar, Hasnan& Ali, Abdul Manaf…[et al.]. Viability Reduction and Rac1 Gene Downregulation of Heterogeneous Ex-Vivo Glioma Acute Slice Infected by the Oncolytic Newcastle Disease Virus Strain V4UPM. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030226