Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for Assaying Leishmania infantum Antibodies in Dogs
Joint Authors
Proverbio, Daniela
Spada, Eva
Baggiani, Luciana
Perego, Roberta
Bagnagatti de Giorgi, Giada
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This study compares a rapid Immunospecific Kalazar Canine Rapid Spot IF with the gold standard test (indirect fluorescent antibody test (IFAT)) for detection of Leishmania infantum specific IgG serum antibodies in naturally exposed dogs.
Serum samples were obtained from 89 healthy dogs and dogs affected by canine leishmaniosis (CanL).
IgG-IFAT titers ≥80 were considered positive.
Anti-L.
infantum IgG antibodies were found in 54 samples with titers ranging from 1 : 80 to 1 : 5120.
The performance of the rapid Immunospecific Kalazar was evaluated using a ROC curve.
The area under the ROC curve of 0.957 was significantly different from 0.5 (P<0.0001), and therefore it can be concluded that the rapid Immunospecific Kalazar has the ability to distinguish canine sera with and without L.
infantum IgG.
The best performance of the test was at a cutoff >0 (sensitivity 92.6%, specificity 97%).
The test can be used for disease screening if the cutoff is >0 (highest sensitivity, 92.6%) and is recommended as confirmatory test for the presence of L.
infantum IgG antibodies if the cutoff is set >2 (highest specificity, 100%).
American Psychological Association (APA)
Proverbio, Daniela& Spada, Eva& Baggiani, Luciana& Bagnagatti de Giorgi, Giada& Perego, Roberta. 2013. Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for Assaying Leishmania infantum Antibodies in Dogs. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030229
Modern Language Association (MLA)
Proverbio, Daniela…[et al.]. Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for Assaying Leishmania infantum Antibodies in Dogs. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1030229
American Medical Association (AMA)
Proverbio, Daniela& Spada, Eva& Baggiani, Luciana& Bagnagatti de Giorgi, Giada& Perego, Roberta. Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for Assaying Leishmania infantum Antibodies in Dogs. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030229
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030229