A Flow Cytometric Analysis of Vitreous Inflammatory Cells in Patients with Proliferative Diabetic Retinopathy
Joint Authors
Urbančič, Mojca
Kloboves Prevodnik, Veronika
Globočnik Petrovič, Mojca
Petrovič, Daniel
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The purpose of this study was to investigate inflammatory cells in vitreous from patients with proliferative diabetic retinopathy (PDR) using flow cytometric analysis.
Twenty-eight patients with PDR requiring vitrectomy because of macular traction or tractional retinal detachment were enrolled in the study (n=28), and 6 patients with macular hole (MH) formed the control group.
Samples of vitreous and peripheral venous blood were obtained at the beginning of vitrectomy.
T lymphocytes were found in vitreous from patients with PDR, and CD4/CD8 ratio was higher in vitreous (median 4.3) compared to blood (median 1.9; P=0.003).
No B lymphocytes were detected in vitreous.
The percentage of histiocytes/macrophages was significantly higher in vitreous (median 62.1) in comparison with blood (median 5.5; P<0.0001).
No lymphocytes were detected in vitreous of the control group.
There were more T lymphocytes in vitreous from patients with active PDR.
No association between cells in the vitreous and visual acuity improvement after surgery was found.
In conclusion, T lymphocytes are found in vitreous from patients with PDR and reflect the activity of PDR but do not seem to predict visual prognosis.
Higher CD4/CD8 ratio in vitreous compared to blood from patients with PDR is consistent with local inflammatory response in PDR.
American Psychological Association (APA)
Urbančič, Mojca& Kloboves Prevodnik, Veronika& Petrovič, Daniel& Globočnik Petrovič, Mojca. 2013. A Flow Cytometric Analysis of Vitreous Inflammatory Cells in Patients with Proliferative Diabetic Retinopathy. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030238
Modern Language Association (MLA)
Urbančič, Mojca…[et al.]. A Flow Cytometric Analysis of Vitreous Inflammatory Cells in Patients with Proliferative Diabetic Retinopathy. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030238
American Medical Association (AMA)
Urbančič, Mojca& Kloboves Prevodnik, Veronika& Petrovič, Daniel& Globočnik Petrovič, Mojca. A Flow Cytometric Analysis of Vitreous Inflammatory Cells in Patients with Proliferative Diabetic Retinopathy. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030238
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030238