Mesenchymal Stem Cell-Like Cells Derived from Mouse Induced Pluripotent Stem Cells Ameliorate Diabetic Polyneuropathy in Mice
Joint Authors
Himeno, Tatsuhito
Kamiya, Hideki
Naruse, Keiko
Cheng, Zhao
Kondo, Masaki
Okawa, Tetsuji
Fujiya, Atsushi
Kato, Jiro
Suzuki, Hirohiko
Kito, Tetsutaro
Hamada, Yoji
Oiso, Yutaka
Nakamura, Jiro
Isobe, Ken-ichi
Ito, Sachiko
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Although pathological involvements of diabetic polyneuropathy (DPN) have been reported, no dependable treatment of DPN has been achieved.
Recent studies have shown that mesenchymal stem cells (MSCs) ameliorate DPN.
Here we demonstrate a differentiation of induced pluripotent stem cells (iPSCs) into MSC-like cells and investigate the therapeutic potential of the MSC-like cell transplantation on DPN.
Research Design and Methods.
For induction into MSC-like cells, GFP-expressing iPSCs were cultured with retinoic acid, followed by adherent culture for 4 months.
The MSC-like cells, characterized with flow cytometry and RT-PCR analyses, were transplanted into muscles of streptozotocin-diabetic mice.
Three weeks after the transplantation, neurophysiological functions were evaluated.
Results.
The MSC-like cells expressed MSC markers and angiogenic/neurotrophic factors.
The transplanted cells resided in hindlimb muscles and peripheral nerves, and some transplanted cells expressed S100β in the nerves.
Impairments of current perception thresholds, nerve conduction velocities, and plantar skin blood flow in the diabetic mice were ameliorated in limbs with the transplanted cells.
The capillary number-to-muscle fiber ratios were increased in transplanted hindlimbs of diabetic mice.
Conclusions.
These results suggest that MSC-like cell transplantation might have therapeutic effects on DPN through secreting angiogenic/neurotrophic factors and differentiation to Schwann cell-like cells.
American Psychological Association (APA)
Himeno, Tatsuhito& Kamiya, Hideki& Naruse, Keiko& Cheng, Zhao& Ito, Sachiko& Kondo, Masaki…[et al.]. 2013. Mesenchymal Stem Cell-Like Cells Derived from Mouse Induced Pluripotent Stem Cells Ameliorate Diabetic Polyneuropathy in Mice. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030251
Modern Language Association (MLA)
Himeno, Tatsuhito…[et al.]. Mesenchymal Stem Cell-Like Cells Derived from Mouse Induced Pluripotent Stem Cells Ameliorate Diabetic Polyneuropathy in Mice. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030251
American Medical Association (AMA)
Himeno, Tatsuhito& Kamiya, Hideki& Naruse, Keiko& Cheng, Zhao& Ito, Sachiko& Kondo, Masaki…[et al.]. Mesenchymal Stem Cell-Like Cells Derived from Mouse Induced Pluripotent Stem Cells Ameliorate Diabetic Polyneuropathy in Mice. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030251
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030251