Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration
Joint Authors
Ito, Yoshihiro
Son, Tae Il
Kang, Jeonghwa
Tada, Seiichi
Kitajima, Takashi
Aigaki, Toshiro
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Titanium was treated with 3,4-dihydroxy-L-phenylalanine (DOPA) or dopamine to immobilize bone morphogenetic protein-2 (BMP2), a biomolecule.
DOPA and dopamine solutions turned into suspensions, and precipitates were produced at high pH.
Both treatments produced a brown surface on titanium that was thicker at high pH than low pH.
Dopamine produced a thicker layer than DOPA.
The hydrophobicity of the surfaces increased after treatment with dopamine independent of pH.
Furthermore, there were more amino groups in the layers formed at pH 8.5 than pH 4.5 in both treatments.
Dopamine treatment produced more amino groups in the layer than DOPA.
BMP2 was immobilized on the treated surfaces via a coupling reaction using carbodiimide.
More BMP2 was immobilized on surfaces treated at pH 8.5 than pH 4.5 in both treatments.
The immobilized BMP induced specific signal transduction and alkali phosphatase, a differentiation marker.
Thus, the present study demonstrates that titanium treated with DOPA or dopamine can become bioactive via the surface immobilization of BMP2, which induces specific signal transduction.
American Psychological Association (APA)
Kang, Jeonghwa& Tada, Seiichi& Kitajima, Takashi& Son, Tae Il& Aigaki, Toshiro& Ito, Yoshihiro. 2013. Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030261
Modern Language Association (MLA)
Kang, Jeonghwa…[et al.]. Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1030261
American Medical Association (AMA)
Kang, Jeonghwa& Tada, Seiichi& Kitajima, Takashi& Son, Tae Il& Aigaki, Toshiro& Ito, Yoshihiro. Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030261
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030261