![](/images/graphics-bg.png)
In Vitro Effects of Low-Intensity Pulsed Ultrasound Stimulation on the Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells for Tooth Tissue Engineering
Joint Authors
Kim, Jangho
Choung, Pill-Hoon
Park, Soo Hyun
Seonwoo, Hoon
Chung, Jong Hoon
Lim, Ki Taek
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-30
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Ultrasound stimulation produces significant multifunctional effects that are directly relevant to alveolar bone formation, which is necessary for periodontal healing and regeneration.
We focused to find out effects of specific duty cycles and the percentage oftime that ultrasound is being generated over one on/off pulse period, under ultrasound stimulation.
Low-intensity pulsed ultrasound ((LIPUS) 1 MHz) with duty cycles of 20% and 50% was used in this study, and human alveolar bone-derived mesenchymal stem cells (hABMSCs) were treated with an intensity of 50 mW/cm2 and exposure time of 10 min/day.
hABMSCs exposed at duty cycles of 20% and 50% had similar cell viability (O.D.), which was higher (*P<0.05) than that of control cells.
The alkaline phosphatase (ALP) was significantly enhanced at 1 week with LIPUS treatment in osteogenic cultures as compared to control.
Gene expressions showed significantly higher expression levels of CD29, CD44, COL1, and OCN in the hABMSCs under LIPUS treatment when compared to control after two weeks of treatment.
The effects were partially controlled by LIPUS treatment, indicating that modulation of osteogenesis in hABMSCs was related to the specific stimulation.
Furthermore, mineralized noduleformation was markedly increased after LIPUS treatment than that seen in untreated cells.
Through simple staining methods such as Alizarin red and von Kossa staining, calcium deposits generated their highest levels at about 3 weeks.
These results suggestthat LIPUS could enhance the cell viability and osteogenic differentiation of hABMSCs, and could be part of effective treatment methods for clinical applications.
American Psychological Association (APA)
Lim, Ki Taek& Kim, Jangho& Seonwoo, Hoon& Park, Soo Hyun& Choung, Pill-Hoon& Chung, Jong Hoon. 2013. In Vitro Effects of Low-Intensity Pulsed Ultrasound Stimulation on the Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells for Tooth Tissue Engineering. BioMed Research International،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1030266
Modern Language Association (MLA)
Lim, Ki Taek…[et al.]. In Vitro Effects of Low-Intensity Pulsed Ultrasound Stimulation on the Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells for Tooth Tissue Engineering. BioMed Research International No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-1030266
American Medical Association (AMA)
Lim, Ki Taek& Kim, Jangho& Seonwoo, Hoon& Park, Soo Hyun& Choung, Pill-Hoon& Chung, Jong Hoon. In Vitro Effects of Low-Intensity Pulsed Ultrasound Stimulation on the Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells for Tooth Tissue Engineering. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1030266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030266