Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage in Urtica dioica
Joint Authors
Gjorgieva, Darinka
Kadifkova Panovska, Tatjana
Bačeva, Katerina
Ruskovska, Tatjana
Stafilov, T.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Heavy metals have the potential to interact and induce several stress responses in the plants; thus, effects of heavy metal stress on DNA damages and total antioxidants level in Urtica dioica leaves and stems were investigated.
The samples are sampled from areas with different metal exposition.
Metal content was analyzed by Inductively Coupled Plasma-Atomic Emission Spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay was used, and genomic DNA isolation from frozen plant samples was performed to obtain DNA fingerprints of investigated plant.
It was found that heavy metal contents in stems generally changed synchronously with those in leaves of the plant, and extraneous metals led to imbalance of mineral nutrient elements.
DNA damages were investigated by Random Amplified Polymorphic DNA (RAPD) technique, and the results demonstrated that the samples exposed to metals yielded a large number of new fragments (total 12) in comparison with the control sample.
This study showed that DNA stability is highly affected by metal pollution which was identified by RAPD markers.
Results suggested that heavy metal stress influences antioxidant status and also induces DNA damages in U.
dioica which may help to understand the mechanisms of metals genotoxicity.
American Psychological Association (APA)
Gjorgieva, Darinka& Kadifkova Panovska, Tatjana& Ruskovska, Tatjana& Bačeva, Katerina& Stafilov, T.. 2013. Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage in Urtica dioica. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030272
Modern Language Association (MLA)
Gjorgieva, Darinka…[et al.]. Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage in Urtica dioica. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1030272
American Medical Association (AMA)
Gjorgieva, Darinka& Kadifkova Panovska, Tatjana& Ruskovska, Tatjana& Bačeva, Katerina& Stafilov, T.. Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage in Urtica dioica. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030272
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030272