A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth

Abstract EN

A quick foodborne pathogen screening method after six-hour enrichment culture with a broad-range food pathogen enrichment broth is described.

Pathogenic factors of Salmonella enterica, Shigella spp., enteroinvasive Escherichia coli, and enterohemorrhagic E.

coli are amplified with a cocktail primer and rapid polymerase chain reaction (PCR), which finishes amplification in 30 min.

The PCR amplicon was differentiated with a dipstick DNA chromatography assay in 5–10 min.

Starting from a four- to six-hour enrichment culture, this assay was finished within 45 min.

Detection sensitivity of this protocol was less than 2.5 CFU/25 g for S.

enterica and 3.3 CFU/25 g for enterohemorrhagic E.

coli in spiked ground meat experiments.