Validation of New Allele-Specific Real-Time PCR System for Thiopurine Methyltransferase Genotyping in Korean Population
Joint Authors
Chun, Sail
Lee, Woochang
Lee, Hye Won
Min, Won-Ki
Kim, Sollip
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-28
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Introduction.
Thiopurine drugs are metabolized via S-methylation and catalyzed by thiopurine S-methyltransferase (TPMT).
Patients with very low TPMT activity are at high risk of fatal bone marrow toxicity when standard doses of thiopurine drugs are administered.
TPMT genotyping can predict TPMT activity and is not affected by transfusion or red blood cell defects.
Here, we report a new allele-specific real-time polymerase chain reaction (PCR) system for thiopurine methyltransferase genotyping that is validated in Korean population.
Materials and Methods.
Three major TPMT single-nucleotide polymorphisms (TPMT*2, *3B, and *3C) were genotyped using real-time PCR with the allele-specific primers and probes.
Internal positive controls were included in each well, and an automatic interpretative algorithm was applied.
This system was validated using 244 clinical samples and 2 commercial DNA samples that had been previously genotyped using PCR-direct sequencing.
Results.
All of the obtained results are concordant with those of the reference method.
All of the internal positive control reactions were successful.
The allele frequency of TPMT*3C was 2.05% (10 of 488 alleles).
All of the patients with variant alleles were heterozygotes, and no homozygotes were detected.
No TPMT*2, *3A, or *3B alleles were observed in this Korean population.
Conclusion.
This rapid, accurate, and user-friendly genotyping system can be readily used to improve the efficacy and safety of thiopurine treatments in clinical practice.
American Psychological Association (APA)
Kim, Sollip& Lee, Hye Won& Lee, Woochang& Chun, Sail& Min, Won-Ki. 2013. Validation of New Allele-Specific Real-Time PCR System for Thiopurine Methyltransferase Genotyping in Korean Population. BioMed Research International،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1030346
Modern Language Association (MLA)
Kim, Sollip…[et al.]. Validation of New Allele-Specific Real-Time PCR System for Thiopurine Methyltransferase Genotyping in Korean Population. BioMed Research International No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-1030346
American Medical Association (AMA)
Kim, Sollip& Lee, Hye Won& Lee, Woochang& Chun, Sail& Min, Won-Ki. Validation of New Allele-Specific Real-Time PCR System for Thiopurine Methyltransferase Genotyping in Korean Population. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1030346
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030346