Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction
Joint Authors
Kongkham, Supranee
Khositseth, Sookkasem
Tasanarong, Adis
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This study aims to investigate the renoprotective effect of recombinant human erythropoietin (rhEPO) treatment could preserve tubular epithelial cell regeneration and ameliorate renal fibrosis by dual inhibition of stress-induced senescence and EMT in unilateral ureteric obstruction (UUO) mouse model.
UUO or sham-operated mice were randomly assigned to receive rhEPO or vehicle treatment and were sacrificed on days 3, 7, and 14.
Kidney specimens were fixed for histopathological and immunohistochemical study.
The expression of S100A4, TGF-β1, BMP-7, Smad2/3, Smad1/5/8, and p16INK4a was determined by western blot and real-time RT-PCR.
Vehicle treated UUO mice had increased tubular atrophy and interstitial fibrosis within 3 to 14 days.
An increase in TGF-β1, Smad2/3, S100A4, and p16INK4a expression and a decrease in BMP-7 and Smad1/5/8 expression were observed in the obstructed kidneys.
p16INK4a was positively correlated with TGF-β1/Smad2/3 and negatively correlated with BMP-7/Smad1/5/8 in UUO mice.
rhEPO treatment significantly suppressed the upregulation of TGF-β, Smad2/3, S100A4, and p16INK4a and preserved the downregulation of BMP-7 and Smad1/5/8, resulting in markedly reduced TA/IF compared to the vehicle treated mice.
The renoprotective effects of rhEPO could ameliorate renal TA/IF by modulating senescence and EMT which could be a part of therapeutic option in patients with chronic kidney disease.
American Psychological Association (APA)
Tasanarong, Adis& Kongkham, Supranee& Khositseth, Sookkasem. 2013. Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030348
Modern Language Association (MLA)
Tasanarong, Adis…[et al.]. Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030348
American Medical Association (AMA)
Tasanarong, Adis& Kongkham, Supranee& Khositseth, Sookkasem. Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030348
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030348