Effects of DL-Homocysteine Thiolactone on Cardiac Contractility, Coronary Flow, and Oxidative Stress Markers in the Isolated Rat Heart: The Role of Different Gasotransmitters

Joint Authors

Pecháňová, Olga
Barudzic, Nevena S.
Srejovic, Ivan
Joksimovic, Jovana
Selakovic, Dragica
Jakovljevic, Vladimir Lj.
Djuric, Dragan M.
Zivkovic, Vladimir I.

Source

BioMed Research International

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-11-24

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

Considering the adverse effects of DL-homocysteine thiolactone hydrochloride (DL-Hcy TLHC) on vascular function and the possible role of oxidative stress in these mechanisms, the aim of this study was to assess the influence of DL-Hcy TLHC alone and in combination with specific inhibitors of important gasotransmitters, such as L-NAME, DL-PAG, and PPR IX, on cardiac contractility, coronary flow, and oxidative stress markers in an isolated rat heart.

The hearts were retrogradely perfused according to the Langendorff technique at a 70 cm H2O and administered 10 μM DL-Hcy TLHC alone or in combination with 30 μM L-NAME, 10 μM DL-PAG, or 10 μM PPR IX.

The following parameters were measured: dp/dt max, dp/dt min, SLVP, DLVP, MBP, HR, and CF.

Oxidative stress markers were measured spectrophotometrically in coronary effluent through TBARS, NO2, O2-, and H2O2 concentrations.

The administration of DL-Hcy TLHC alone decreased dp/dt max, SLVP, and CF but did not change any oxidative stress parameters.

DL-Hcy TLHC with L-NAME decreased CF, O2-, H2O2, and TBARS.

The administration of DL-Hcy TLHC with DL-PAG significantly increased dp/dt max but decreased DLVP, CF, and TBARS.

Administration of DL-Hcy TLHC with PPR IX caused a decrease in dp/dt max, SLVP, HR, CF, and TBARS.

American Psychological Association (APA)

Zivkovic, Vladimir I.& Jakovljevic, Vladimir Lj.& Pecháňová, Olga& Srejovic, Ivan& Joksimovic, Jovana& Selakovic, Dragica…[et al.]. 2013. Effects of DL-Homocysteine Thiolactone on Cardiac Contractility, Coronary Flow, and Oxidative Stress Markers in the Isolated Rat Heart: The Role of Different Gasotransmitters. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030374

Modern Language Association (MLA)

Zivkovic, Vladimir I.…[et al.]. Effects of DL-Homocysteine Thiolactone on Cardiac Contractility, Coronary Flow, and Oxidative Stress Markers in the Isolated Rat Heart: The Role of Different Gasotransmitters. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030374

American Medical Association (AMA)

Zivkovic, Vladimir I.& Jakovljevic, Vladimir Lj.& Pecháňová, Olga& Srejovic, Ivan& Joksimovic, Jovana& Selakovic, Dragica…[et al.]. Effects of DL-Homocysteine Thiolactone on Cardiac Contractility, Coronary Flow, and Oxidative Stress Markers in the Isolated Rat Heart: The Role of Different Gasotransmitters. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030374

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1030374