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DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγNFκB Pathway in C2C12 Myotubes
Joint Authors
Lin, Qiaowei
Zheng, Pei-pei
Zhang, Jian-song
Huang, Feiruo
Wang, Yue
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study was conducted to evaluate the mechanism by which n-3 PUFA regulated the protein degradation in C2C12 myotubes.
Compared with the BSA control, EPA at concentrations from 400 to 600 µM decreased total protein degradation (P<0.01).
However, the total protein degradation was decreased when the concentrations of DHA ranged from 300 µM to 700 µM (P<0.01).
DHA (400 µM, 24 h) more efficiently decreased the IκBα phosphorylation and increased in the IκBα protein level than 400 µM EPA (P<0.01).
Compared with BSA, 400 µM EPA and DHA resulted in a 47% or 68% induction of the NFκB DNA binding activity, respectively (P<0.01).
Meanwhile, 400 µM EPA and DHA resulted in a 1.3-fold and 2.0-fold induction of the PPARγ expression, respectively (P<0.01).
In C2C12 myotubes for PPARγ knockdown, neither 400 µM EPA nor DHA affected the levels of p-IκBα, total IκBα or NFκB DNA binding activity compared with BSA (P>0.05).
Interestingly, EPA and DHA both still decreased the total protein degradation, although PPARγ knockdown attenuated the suppressive effects of EPA and DHA on the total protein degradation (P<0.01).
These results revealed that DHA inhibits protein degradation more efficiently than EPA by regulating the PPARγ/NF-κB pathway in C2C12 myotubes.
American Psychological Association (APA)
Wang, Yue& Lin, Qiaowei& Zheng, Pei-pei& Zhang, Jian-song& Huang, Feiruo. 2013. DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγNFκB Pathway in C2C12 Myotubes. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030380
Modern Language Association (MLA)
Wang, Yue…[et al.]. DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγNFκB Pathway in C2C12 Myotubes. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030380
American Medical Association (AMA)
Wang, Yue& Lin, Qiaowei& Zheng, Pei-pei& Zhang, Jian-song& Huang, Feiruo. DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγNFκB Pathway in C2C12 Myotubes. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030380
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030380