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Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix
Joint Authors
Choi, Jung Hoon
Park, Young Hyun
Yun, Jung Im
Han, Na Rae
Park, Hye Jin
Ahn, Ji Yeon
Kim, Choonghyo
Lee, Eunsong
Lim, Jeong Mook
Lee, Seung Tae
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Although preparation of early-stage bone-marrow-derived mesenchymal stem cells (BM-MSCs) is critical for successful cell transplantation therapy, no culture system offers a sufficient number of early-stage BM-MSCs for cell transplantation.
Accordingly, we developed a culture system capable of producing a large number of early-stage BM-MSCs by using gelatin-coated matrix.
The greatest retrieval and proliferation rates of the earliest-stage rat BM-MSCs were detected in bone-marrow-derived cells cultured on 1% (wt/v) gelatin-coated matrix, which showed significantly greater colony forming unit-fibroblast number, diameter, and total cell number.
Moreover, continuous culture of the earliest-stage BM-MSCs on 1% (wt/v) gelatin-coated matrix resulted in a maximum of 21.2±2.7 fold increase in the cumulative total number of early-stage BM-MSCs at passage 5.
BM-MSCs generated in large quantities due to a reduced doubling time and an increased yield of cell population in S/G2/M phase showed typical fibroblast-like morphology and no significant differences in BM-MSC-related surface marker expression and differentiation potential, except for an increased ratio of differentiation into a neurogenic lineage.
The use of gelatin-coated matrix in the retrieval and culture of BM-MSCs contributes greatly to the effective isolation and mass production of early-stage BM-MSCs.
American Psychological Association (APA)
Park, Young Hyun& Yun, Jung Im& Han, Na Rae& Park, Hye Jin& Ahn, Ji Yeon& Kim, Choonghyo…[et al.]. 2013. Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030419
Modern Language Association (MLA)
Park, Young Hyun…[et al.]. Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030419
American Medical Association (AMA)
Park, Young Hyun& Yun, Jung Im& Han, Na Rae& Park, Hye Jin& Ahn, Ji Yeon& Kim, Choonghyo…[et al.]. Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030419
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030419