Spectroscopic Studies on Unfolding Processes of Apo-Neuroglobin Induced by Guanidine Hydrochloride and Urea
Joint Authors
Gao, Chaohui
Zhang, Cui
Mu, Jianshuai
Qiu, Zhanglei
Li, Lianzhi
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Neuroglobin (Ngb), a recently discovered globin, is predominantly expressed in the brain, retina, and other nerve tissues of vertebrates.
The unfolding processes of apo-neuroglobin (apoNgb) induced by guanidine hydrochloride (GdnHCl) and urea were investigated by spectroscopic methods.
In the unfolding processes, apoNgb's tertiary structural transition was monitored by the changes of intrinsic fluorescence emission spectra, and its secondary structural transition was measured by the changes of far-ultraviolet circular dichroism (CD) spectra.
In addition, 8-anilino-1-naphthalenesulfonic acid (ANS), a hydrophobic cluster binding dye, was also used to monitor the unfolding process of apoNgb and to explore its intermediates.
Results showed that GdnHCl-induced unfolding of apoNgb was via a three-state pathway, that is, Native state (N) → Intermediate state (I) → Unfolded state (U), during which the intermediate was inferred by an increase in fluorescence intensity and the change of CD value.
Gibbs free energy changes are 10.2 kJ·mol−1 for the first unfolding transition and 14.0 kJ·mol−1 for the second transition.
However, urea-induced unfolding of apoNgb only underwent a two-state transition: Native state (N) → Partially unfolded state (P).
The result showed that GdnHCl can efficiently affect the conformational states of apoNgb compared with those of urea.
The work will benefit to have an understanding of the unfolding mechanism of apoNgb induced by GdnHCl and urea.
American Psychological Association (APA)
Zhang, Cui& Gao, Chaohui& Mu, Jianshuai& Qiu, Zhanglei& Li, Lianzhi. 2013. Spectroscopic Studies on Unfolding Processes of Apo-Neuroglobin Induced by Guanidine Hydrochloride and Urea. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030424
Modern Language Association (MLA)
Zhang, Cui…[et al.]. Spectroscopic Studies on Unfolding Processes of Apo-Neuroglobin Induced by Guanidine Hydrochloride and Urea. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030424
American Medical Association (AMA)
Zhang, Cui& Gao, Chaohui& Mu, Jianshuai& Qiu, Zhanglei& Li, Lianzhi. Spectroscopic Studies on Unfolding Processes of Apo-Neuroglobin Induced by Guanidine Hydrochloride and Urea. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030424
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030424