Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth
Joint Authors
Asmawi, Mohd Zaini
Yam, Mun Fei
Lim, Chung Pin
Fung Ang, Lee
Por, Lip Yee
Wong, Siew Tung
Ahmad, Mariam
Basir, Rusliza
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The present study evaluated the antioxidant activity and potential toxicity of 50% methanolic extract of Orthosiphon stamineus (Lamiaceae) leaves (MEOS) after acute and subchronic administration in rats.
Superoxide radical scavenging, hydroxyl radical scavenging, and ferrous ion chelating methods were used to evaluate the antioxidant properties of the extract.
In acute toxicity study, single dose of MEOS, 5000 mg/kg, was administered to rats by oral gavage, and the treated rats were monitored for 14 days.
While in the subchronic toxicity study, MEOS was administered orally, at doses of 1250, 2500, and 5000 mg/kg/day for 28 days.
From the results, MEOS showed good superoxide radical scavenging, hydroxyl radical scavenging, ferrous ion chelating, and antilipid peroxidation activities.
There was no mortality detected or any signs of toxicity in acute and subchronic toxicity studies.
Furthermore, there was no significant difference in bodyweight, relative organ weight, and haematological and biochemical parameters between both male and female treated rats in any doses tested.
No abnormality of internal organs was observed between treatment and control groups.
The oral lethal dose determined was more than 5000 mg/kg and the no-observed-adverse-effect level (NOAEL) of MEOS for both male and female rats is considered to be 5000 mg/kg per day.
American Psychological Association (APA)
Yam, Mun Fei& Lim, Chung Pin& Fung Ang, Lee& Por, Lip Yee& Wong, Siew Tung& Asmawi, Mohd Zaini…[et al.]. 2013. Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030426
Modern Language Association (MLA)
Yam, Mun Fei…[et al.]. Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030426
American Medical Association (AMA)
Yam, Mun Fei& Lim, Chung Pin& Fung Ang, Lee& Por, Lip Yee& Wong, Siew Tung& Asmawi, Mohd Zaini…[et al.]. Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030426
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030426