Cardiac Fibroblast-Derived Extracellular Matrix (Biomatrix) as a Model for the Studies of Cardiac Primitive Cell Biological Properties in Normal and Pathological Adult Human Heart
Joint Authors
Della Corte, Alessandro
Meglio, Franca Di
Castaldo, Clotilde
Nurzynska, Daria
Miraglia, Rita
Sacco, Anna Maria
Romano, Veronica
Bancone, Ciro
Montagnani, Stefania
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cardiac tissue regeneration is guided by stem cells and their microenvironment.
It has been recently described that both cardiac stem/primitive cells and extracellular matrix (ECM) change in pathological conditions.
This study describes the method for the production of ECM typical of adult human heart in the normal and pathological conditions (ischemic heart disease) and highlights the potential use of cardiac fibroblast-derived ECM for in vitro studies of the interactions between ECM components and cardiac primitive cells responsible for tissue regeneration.
Fibroblasts isolated from adult human normal and pathological heart with ischemic cardiomyopathy were cultured to obtain extracellular matrix (biomatrix), composed of typical extracellular matrix proteins, such as collagen and fibronectin, and matricellular proteins, laminin, and tenascin.
After decellularization, this substrate was used to assess biological properties of cardiac primitive cells: proliferation and migration were stimulated by biomatrix from normal heart, while both types of biomatrix protected cardiac primitive cells from apoptosis.
Our model can be used for studies of cell-matrix interactions and help to determine the biochemical cues that regulate cardiac primitive cell biological properties and guide cardiac tissue regeneration.
American Psychological Association (APA)
Castaldo, Clotilde& Meglio, Franca Di& Miraglia, Rita& Sacco, Anna Maria& Romano, Veronica& Bancone, Ciro…[et al.]. 2013. Cardiac Fibroblast-Derived Extracellular Matrix (Biomatrix) as a Model for the Studies of Cardiac Primitive Cell Biological Properties in Normal and Pathological Adult Human Heart. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030429
Modern Language Association (MLA)
Castaldo, Clotilde…[et al.]. Cardiac Fibroblast-Derived Extracellular Matrix (Biomatrix) as a Model for the Studies of Cardiac Primitive Cell Biological Properties in Normal and Pathological Adult Human Heart. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030429
American Medical Association (AMA)
Castaldo, Clotilde& Meglio, Franca Di& Miraglia, Rita& Sacco, Anna Maria& Romano, Veronica& Bancone, Ciro…[et al.]. Cardiac Fibroblast-Derived Extracellular Matrix (Biomatrix) as a Model for the Studies of Cardiac Primitive Cell Biological Properties in Normal and Pathological Adult Human Heart. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030429
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030429