Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane
Joint Authors
You-Xiong, Que
Liu, Jing
Guo, Jinlong
Chen, Rukai
Grisham, Michael Paul
Li-Ping, Xu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Ratoon stunt, caused by the xylem-limited coryneform bacterium Leifsonia xyli subsp.
xyli (Lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries.
Based on loop-mediated isothermal amplification (LAMP), we developed a method for detecting Lxx.
The major advantages of the LAMP method are visual judgment by color and time saving with only 60 min for identification of Lxx and without the need for costly PCR apparatus and gel scanner.
In the present study, positive and negative samples detected by the LAMP method were clearly distinguishable.
When total DNA extracted from internode juice was used as the template, the sensitivity of LAMP was 10 times higher than that of the conventional PCR detection.
The LAMP assay is a highly specific, rapid, and sensitive method for the diagnosis of ratoon stunt caused by Lxx in sugarcane.
This is the first report of LAMP-based assay for the detection of Lxx in sugarcane.
American Psychological Association (APA)
Liu, Jing& Li-Ping, Xu& Guo, Jinlong& Chen, Rukai& Grisham, Michael Paul& You-Xiong, Que. 2013. Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030434
Modern Language Association (MLA)
Liu, Jing…[et al.]. Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030434
American Medical Association (AMA)
Liu, Jing& Li-Ping, Xu& Guo, Jinlong& Chen, Rukai& Grisham, Michael Paul& You-Xiong, Que. Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030434
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030434