Kinetics of Molybdenum Reduction to Molybdenum Blue by Bacillus sp. Strain A.rzi
Joint Authors
Halmi, M. I. E.
Shukor, Mohd Yunus
Othman, A. R.
Bakar, N. A.
Johari, W. L. W.
Jirangon, H.
Syed, M. A.
Ahmad, Siti Aqlima
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Molybdenum is very toxic to agricultural animals.
Mo-reducing bacterium can be used to immobilize soluble molybdenum to insoluble forms, reducing its toxicity in the process.
In this work the isolation of a novel molybdate-reducing Gram positive bacterium tentatively identified as Bacillus sp.
strain A.rzi from a metal-contaminated soil is reported.
The cellular reduction of molybdate to molybdenum blue occurred optimally at 4 mM phosphate, using 1% (w/v) glucose, 50 mM molybdate, between 28 and 30°C and at pH 7.3.
The spectrum of the Mo-blue product showed a maximum peak at 865 nm and a shoulder at 700 nm.
Inhibitors of bacterial electron transport system (ETS) such as rotenone, sodium azide, antimycin A, and potassium cyanide could not inhibit the molybdenum-reducing activity.
At 0.1 mM, mercury, copper, cadmium, arsenic, lead, chromium, cobalt, and zinc showed strong inhibition on molybdate reduction by crude enzyme.
The best model that fitted the experimental data well was Luong followed by Haldane and Monod.
The calculated value for Luong’s constants pmax, Ks, Sm, and n was 5.88 μmole Mo-blue hr−1, 70.36 mM, 108.22 mM, and 0.74, respectively.
The characteristics of this bacterium make it an ideal tool for bioremediation of molybdenum pollution.
American Psychological Association (APA)
Othman, A. R.& Bakar, N. A.& Halmi, M. I. E.& Johari, W. L. W.& Ahmad, Siti Aqlima& Jirangon, H.…[et al.]. 2013. Kinetics of Molybdenum Reduction to Molybdenum Blue by Bacillus sp. Strain A.rzi. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030445
Modern Language Association (MLA)
Othman, A. R.…[et al.]. Kinetics of Molybdenum Reduction to Molybdenum Blue by Bacillus sp. Strain A.rzi. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030445
American Medical Association (AMA)
Othman, A. R.& Bakar, N. A.& Halmi, M. I. E.& Johari, W. L. W.& Ahmad, Siti Aqlima& Jirangon, H.…[et al.]. Kinetics of Molybdenum Reduction to Molybdenum Blue by Bacillus sp. Strain A.rzi. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030445
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030445