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Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances
Joint Authors
Slepička, Petr
Slepičková Kasálková, Nikola
Bačáková, Lucie
Švorčík, Václav
Parizek, Martin
Svindrych, Zdenek
Bacakova, Marketa
Lisa, Vera
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-24
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
The attractiveness of synthetic polymers for cell colonization can be affected by physical, chemical, and biological modification of the polymer surface.
In this study, low-density polyethylene (LDPE) was treated by an Ar+ plasma discharge and then grafted with biologically active substances, namely, glycine (Gly), polyethylene glycol (PEG), bovine serum albumin (BSA), colloidal carbon particles (C), or BSA+C.
All modifications increased the oxygen content, the wettability, and the surface free energy of the materials compared to the pristine LDPE, but these changes were most pronounced in LDPE with Gly or PEG, where all the three values were higher than in the only plasma-treated samples.
When seeded with vascular smooth muscle cells (VSMCs), the Gly- or PEG-grafted samples increased mainly the spreading and concentration of focal adhesion proteins talin and vinculin in these cells.
LDPE grafted with BSA or BSA+C showed a similar oxygen content and similar wettability, as the samples only treated with plasma, but the nano- and submicron-scale irregularities on their surface were more pronounced and of a different shape.
These samples promoted predominantly the growth, the formation of a confluent layer, and phenotypic maturation of VSMC, demonstrated by higher concentrations of contractile proteins alpha-actin and SM1 and SM2 myosins.
Thus, the behavior of VSMC on LDPE can be regulated by the type of bioactive substances that are grafted.
American Psychological Association (APA)
Parizek, Martin& Slepičková Kasálková, Nikola& Bačáková, Lucie& Svindrych, Zdenek& Slepička, Petr& Bacakova, Marketa…[et al.]. 2013. Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances. BioMed Research International،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1030446
Modern Language Association (MLA)
Parizek, Martin…[et al.]. Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances. BioMed Research International No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-1030446
American Medical Association (AMA)
Parizek, Martin& Slepičková Kasálková, Nikola& Bačáková, Lucie& Svindrych, Zdenek& Slepička, Petr& Bacakova, Marketa…[et al.]. Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1030446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030446