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Salamander-Derived, Human-Optimized nAG Protein Suppresses Collagen Synthesis and Increases Collagen Degradation in Primary Human Fibroblasts
Joint Authors
al-Qattan, Mohammad M.
Shier, Medhat K.
Abd-Al Wahed, Mervat M.
Mawlana, Ola H.
El-Wetidy, Mohammed S.
Bagayawa, Reginald S.
Ali, Hebatallah H.
Al-Nbaheen, May S.
Aldahmash, Abdullah
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Unlike humans, salamanders regrow their amputated limbs.
Regeneration depends on the presence of regenerating axons which upregulate the expression of newt anterior gradient (nAG) protein.
We had the hypothesis that nAG might have an inhibitory effect on collagen production since excessive collagen production results in scarring, which is a major enemy to regeneration.
nAG gene was designed, synthesized, and cloned.
The cloned vector was then transfected into primary human fibroblasts.
The results showed that the expression of nAG protein in primary human fibroblast cells suppresses the expression of collagen I and III, with or without TGF-β1 stimulation.
This suppression is due to a dual effect of nAG both by decreasing collagen synthesis and by increasing collagen degradation.
Furthermore, nAG had an inhibitory effect on proliferation of transfected fibroblasts.
It was concluded that nAG suppresses collagen through multiple effects.
American Psychological Association (APA)
al-Qattan, Mohammad M.& Shier, Medhat K.& Abd-Al Wahed, Mervat M.& Mawlana, Ola H.& El-Wetidy, Mohammed S.& Bagayawa, Reginald S.…[et al.]. 2013. Salamander-Derived, Human-Optimized nAG Protein Suppresses Collagen Synthesis and Increases Collagen Degradation in Primary Human Fibroblasts. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030476
Modern Language Association (MLA)
al-Qattan, Mohammad M.…[et al.]. Salamander-Derived, Human-Optimized nAG Protein Suppresses Collagen Synthesis and Increases Collagen Degradation in Primary Human Fibroblasts. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030476
American Medical Association (AMA)
al-Qattan, Mohammad M.& Shier, Medhat K.& Abd-Al Wahed, Mervat M.& Mawlana, Ola H.& El-Wetidy, Mohammed S.& Bagayawa, Reginald S.…[et al.]. Salamander-Derived, Human-Optimized nAG Protein Suppresses Collagen Synthesis and Increases Collagen Degradation in Primary Human Fibroblasts. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030476