![](/images/graphics-bg.png)
Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology
Joint Authors
Roma, Cristin
Esposito, Claudia
Rachiglio, Anna Maria
Pasquale, Raffaella
Iannaccone, Alessia
Chicchinelli, Nicoletta
Mancini, Rita
Pisconti, Salvatore
De Luca, Antonella
Botti, Gerardo
Morabito, Alessandro
Franco, Renato
Normanno, Nicola
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Epidermal growth factor receptor (EGFR) mutations in non-small-cell lung cancer (NSCLC) are predictive of response to treatment with tyrosine kinase inhibitors.
Competitive Allele-Specific TaqMan PCR (castPCR) is a highly sensitive and specific technology.
EGFR mutations were assessed by TaqMan Mutation Detection Assays (TMDA) based on castPCR technology in 64 tumor samples: a training set of 30 NSCLC and 6 colorectal carcinoma (CRC) samples and a validation set of 28 NSCLC cases.
The sensitivity and specificity of this method were compared with routine diagnostic techniques including direct sequencing and the EGFR Therascreen RGQ kit.
Analysis of the training set allowed the identification of the threshold value for data analysis (0.2); the maximum cycle threshold (Ct=37); and the cut-off ΔCt value (7) for the EGFR TMDA.
By using these parameters, castPCR technology identified both training and validation set EGFR mutations with similar frequency as compared with the Therascreen kit.
Sequencing detected rare mutations that are not identified by either castPCR or Therascreen, but in samples with low tumor cell content it failed to detect common mutations that were revealed by real-time PCR based methods.
In conclusion, our data suggest that castPCR is highly sensitive and specific to detect EGFR mutations in NSCLC clinical samples.
American Psychological Association (APA)
Roma, Cristin& Esposito, Claudia& Rachiglio, Anna Maria& Pasquale, Raffaella& Iannaccone, Alessia& Chicchinelli, Nicoletta…[et al.]. 2013. Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030482
Modern Language Association (MLA)
Roma, Cristin…[et al.]. Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030482
American Medical Association (AMA)
Roma, Cristin& Esposito, Claudia& Rachiglio, Anna Maria& Pasquale, Raffaella& Iannaccone, Alessia& Chicchinelli, Nicoletta…[et al.]. Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030482