![](/images/graphics-bg.png)
Potential of Chitinolytic Serratia marcescens Strain JPP1 for Biological Control of Aspergillus parasiticus and Aflatoxin
Joint Authors
Yan, Pei-sheng
Wang, Kai
Cao, Li-xin
Ding, Qing-long
Shao, Chi
Zhao, Teng-fei
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Serratia marcescens strain JPP1 was isolated from peanut hulls in Huai'an city, Jiangsu Province, China.
Its potential to inhibit the mycelial growth of Aspergillus parasiticus and the subsequent aflatoxin production was evaluated.
The strain JPP1 could produce chitinase to degrade fungal cell walls, which was the main mechanism of strain JPP1 for biocontrol.
Scanning electron microscopy of fungi treated with the crude chitinase revealed abnormal morphological changes.
While the strain was grown in the peanut hulls-based medium, the chitinase activity reached 7.39 units.
RT-PCR analysis showed that the crude chitinase repressed the transcription of genes involved in the aflatoxin gene cluster, such as aflR, aflC (pksL1), and aflO (dmtA) genes.
By visual agar plate assay and tip culture method, the strain JPP1 exhibited remarkable inhibitory effect on mycelia growth (antifungal ratio >95%) and subsequent aflatoxin production (antiaflatoxigenic ratio >98%).
An in vitro assay with seed coating agent of bacterial suspension showed that strain JPP1 effectively reduced fungal growth and subsequent aflatoxin production on peanut seeds, and its antagonistic effect was superior to the common agricultural fungicide of carbendazim.
These characteristics suggest that S.
marcescens JPP1 strain could potentially be utilized for the biological control of phytopathogenic fungi and aflatoxin in Chinese peanut main producing areas.
American Psychological Association (APA)
Wang, Kai& Yan, Pei-sheng& Cao, Li-xin& Ding, Qing-long& Shao, Chi& Zhao, Teng-fei. 2013. Potential of Chitinolytic Serratia marcescens Strain JPP1 for Biological Control of Aspergillus parasiticus and Aflatoxin. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030507
Modern Language Association (MLA)
Wang, Kai…[et al.]. Potential of Chitinolytic Serratia marcescens Strain JPP1 for Biological Control of Aspergillus parasiticus and Aflatoxin. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030507
American Medical Association (AMA)
Wang, Kai& Yan, Pei-sheng& Cao, Li-xin& Ding, Qing-long& Shao, Chi& Zhao, Teng-fei. Potential of Chitinolytic Serratia marcescens Strain JPP1 for Biological Control of Aspergillus parasiticus and Aflatoxin. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030507
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030507