Crocus sativus L. (Saffron) Stigma Aqueous Extract Induces Apoptosis in Alveolar Human Lung Cancer Cells through Caspase-Dependent Pathways Activation
Joint Authors
Samarghandian, Saeed
Farahmand, Seyed Kazem
Afshari, Reza
Davoodi, Saeideh
Borji, Abasalt
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Worldwide, lung cancer is the most common form of cancer.
Saffron has been used in folk medicine for centuries.
We investigated the potential of saffron to induce cytotoxic and apoptotic effects in lung cancer cells (A549).
We also examined the caspase-dependent pathways activation of saffron-induced apoptosis against the A549 cells.
A549 cells were incubated with different concentrations of saffron extract; then cell morphological changes, cell viability, and apoptosis were determined by the normal invertmicroscope, MTT assay, Annexin V and propidium iodide, and flow cytometric analysis, respectively.
Activated caspases were detected by treatment of saffron in lung cancer cells using fluorescein-labeled inhibitors of polycaspases.
The proliferation of the A549 cells were decreased after treatment with saffron in a dose- and time-dependent manner.
The percentage of apoptotic cells increased with saffron concentrations.
Saffron induced morphological changes, decreased percentage of viable cells, and induced apoptosis.
Saffron could induce apoptosis in the A549 cells and activate caspase pathways.
The levels of caspases involved in saffron-induced apoptosis in the A549 cells indicating caspase-dependent pathway were induced by saffron.
The anticancer activity of the aqueous extract of saffron could be attributed partly to its inhibition of the cell proliferation and induction of apoptosis in cancer cells through caspase-dependent pathways activation.
American Psychological Association (APA)
Samarghandian, Saeed& Borji, Abasalt& Farahmand, Seyed Kazem& Afshari, Reza& Davoodi, Saeideh. 2013. Crocus sativus L. (Saffron) Stigma Aqueous Extract Induces Apoptosis in Alveolar Human Lung Cancer Cells through Caspase-Dependent Pathways Activation. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030530
Modern Language Association (MLA)
Samarghandian, Saeed…[et al.]. Crocus sativus L. (Saffron) Stigma Aqueous Extract Induces Apoptosis in Alveolar Human Lung Cancer Cells through Caspase-Dependent Pathways Activation. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030530
American Medical Association (AMA)
Samarghandian, Saeed& Borji, Abasalt& Farahmand, Seyed Kazem& Afshari, Reza& Davoodi, Saeideh. Crocus sativus L. (Saffron) Stigma Aqueous Extract Induces Apoptosis in Alveolar Human Lung Cancer Cells through Caspase-Dependent Pathways Activation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030530
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030530