Quantitative PCR as an Alternative in the Diagnosis of Long-QT Syndrome
Joint Authors
Moric-Janiszewska, Ewa
Szczurko, Magdalena
Węglarz, Ludmiła
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Congenital long-QT syndrome is a genetic disorder associated with abnormalities in the function and/or structure of cardiac ion channels.
Up to the present, 13 types of the disease have been described (LQTS1-13) which result from the fact that 13 genes of which mutations can have an influence on the occurrence of the disease have been identified.
Characteristic symptoms of the disease include the changes in the ECG (QT interval prolonged above 450 ms), “torsade de pointes,” fainting, and even sudden cardiac death.
The present study has been focused on two types of the disease, namely, LQTS1 and LQTS2.
The examination of two appropriate genes expression (KCNQ1; KCNH2) at the transcription level by QRT-PCR in a group of LQTS patients and a healthy control group showed different transcriptional activities of KCNH2 gene in LQTS2 patients compared to the control individuals.
KCNQ1 gene expression study did not reveal such differences between both groups.
The results indicate that QRT-PCR may serve as a complimentary method to the identification of molecular alterations in genetic determinants of LQTS2 only, but it cannot be used as a sole diagnostic criterion.
American Psychological Association (APA)
Moric-Janiszewska, Ewa& Węglarz, Ludmiła& Szczurko, Magdalena. 2013. Quantitative PCR as an Alternative in the Diagnosis of Long-QT Syndrome. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030532
Modern Language Association (MLA)
Moric-Janiszewska, Ewa…[et al.]. Quantitative PCR as an Alternative in the Diagnosis of Long-QT Syndrome. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030532
American Medical Association (AMA)
Moric-Janiszewska, Ewa& Węglarz, Ludmiła& Szczurko, Magdalena. Quantitative PCR as an Alternative in the Diagnosis of Long-QT Syndrome. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030532
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030532