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Comparison of Explant-Derived and Enzymatic Digestion-Derived MSCs and the Growth Factors from Wharton’s Jelly
Joint Authors
Song, Eun Young
Roh, Eun Youn
Shin, Sue
Yoon, Jong Hyun
Jung, Nam Hee
Chang, Ju Young
Kim, Byoung Jae
Jeon, Hye Won
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Wharton’s jelly is not only one of the most promising tissue sources for mesenchymal stem cells (MSCs) but also a source of natural growth factors.
To prove that we can get both natural growth factors and MSCs from Wharton’s jelly, we compared cellular characteristics and the level of basic fibroblast growth factor (bFGF) from samples using the explant culture method to those derived from the traditional enzymatic culture method.
The levels of bFGF were 27.0±11.7 ng/g on day 3, 15.6±11.1 ng/g on day 6, and decreased to 2.6±1.2 ng/g on day 14.
The total amount of bFGF released was 55.0±25.6 ng/g on explant culture.
Compared with the traditional enzymatic digestion method, the explant culture method showed a tendency to release higher levels of bFGF in supernatant media for the first week of culture, and the higher cellular yield at passage 0 (4.89±3.2×105/g versus 1.75±2.2×105/g, P=0.01).
In addition, the genes related to mitosis were upregulated in the explant-derived MSCs.
American Psychological Association (APA)
Yoon, Jong Hyun& Roh, Eun Youn& Shin, Sue& Jung, Nam Hee& Song, Eun Young& Chang, Ju Young…[et al.]. 2013. Comparison of Explant-Derived and Enzymatic Digestion-Derived MSCs and the Growth Factors from Wharton’s Jelly. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030541
Modern Language Association (MLA)
Yoon, Jong Hyun…[et al.]. Comparison of Explant-Derived and Enzymatic Digestion-Derived MSCs and the Growth Factors from Wharton’s Jelly. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030541
American Medical Association (AMA)
Yoon, Jong Hyun& Roh, Eun Youn& Shin, Sue& Jung, Nam Hee& Song, Eun Young& Chang, Ju Young…[et al.]. Comparison of Explant-Derived and Enzymatic Digestion-Derived MSCs and the Growth Factors from Wharton’s Jelly. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030541
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030541