![](/images/graphics-bg.png)
The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India
Joint Authors
Kangne, Harshada K.
Jijina, Farah F.
Italia, Yazdi M.
Jain, Dipti L.
Gupta, Maya
Mukesh, Rati D.
Colah, Roshan B.
Pradhan, Vandana
Ghosh, Kanjaksha
Nadkarni, Anita
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
Sickle cell disease has variable clinical manifestations.
Activation of neutrophils plays an important role in the initiation and propagation of vaso occlusive crises which can be analysed by determining the expression of neutrophil antigens such as CD16, CD32, and CD62L.
The common FcγR polymorphisms (FcγRIIA and FcγRIIIB) are considered to influence clinical presentation.
This study focuses on distribution of FcγR polymorphisms and their association with neutrophil activity among the patients from western India.
Methods.
In this paper 127 sickle cell anemia patients and 58 patients with sickle-β-thalassemia (median age 12±8.58 years) with variable clinical phenotypes along with 175 normals were investigated.
FcγRs polymorphisms were analysed by RFLP and AS-PCR.
Activation of neutrophils was measured by flow cytometry.
Results.
The genotypic frequency of the H/R genotype of FcγRIIA and the NA1/NA1 genotype of FcγRIIIB was significantly decreased in patients compared to normals (P-0.0074, P-0.0471, resp.).
We found a significant difference in the expression of CD32 and CD62L among the patients as against normals.
A significantly higher expression of CD32 was seen in the milder patients with the H/H genotype (P-0.0231), whereas the expression of CD16 was higher in severe patients with the NA2/NA2 genotype (P-0.0312).
Conclusion.
The two FcγR polymorphisms had significant association with variable phenotypes of sickle cell disease.
The expression of CD62L decreased in our patients indicating activation of neutrophils.
American Psychological Association (APA)
Kangne, Harshada K.& Jijina, Farah F.& Italia, Yazdi M.& Jain, Dipti L.& Nadkarni, Anita& Gupta, Maya…[et al.]. 2013. The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030556
Modern Language Association (MLA)
Kangne, Harshada K.…[et al.]. The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030556
American Medical Association (AMA)
Kangne, Harshada K.& Jijina, Farah F.& Italia, Yazdi M.& Jain, Dipti L.& Nadkarni, Anita& Gupta, Maya…[et al.]. The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030556
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030556