Production of the Quinone-Methide Triterpene Maytenin by In Vitro Adventitious Roots of Peritassa campestris (Cambess.) A.C.Sm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MSMS
Joint Authors
Pereira, Ana Maria S.
Santos, Vânia A. F. F. M.
Paz, Tiago Antunes
Inácio, Marielle Cascaes
Pina, Edieidia Souza
Furlan, Maysa
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Establishment of adventitious root cultures of Peritassa campestris (Celastraceae) was achieved from seed cotyledons cultured in semisolid Woody Plant Medium (WPM) supplemented with 2% sucrose, 0.01% PVP, and 4.0 mg L−1 IBA.
Culture period on accumulation of biomass and quinone-methide triterpene maytenin in adventitious root were investigated.
The accumulation of maytenin in these roots was compared with its accumulation in the roots of seedlings grown in a greenhouse (one year old).
A rapid detection and identification of maytenin by direct injection into an atmospheric-pressure chemical ionization ion trap tandem mass spectrometer (APCI-IT-MS/MS) were performed without prior chromatographic separation.
In vitro, the greatest accumulation of biomass occurred within 60 days of culture.
The highest level of maytenin—972.11 μg·g−1 dry weight—was detected at seven days of cultivation; this value was 5.55-fold higher than that found in the roots of seedlings grown in a greenhouse.
American Psychological Association (APA)
Paz, Tiago Antunes& Santos, Vânia A. F. F. M.& Inácio, Marielle Cascaes& Pina, Edieidia Souza& Pereira, Ana Maria S.& Furlan, Maysa. 2013. Production of the Quinone-Methide Triterpene Maytenin by In Vitro Adventitious Roots of Peritassa campestris (Cambess.) A.C.Sm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MSMS. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030589
Modern Language Association (MLA)
Paz, Tiago Antunes…[et al.]. Production of the Quinone-Methide Triterpene Maytenin by In Vitro Adventitious Roots of Peritassa campestris (Cambess.) A.C.Sm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MSMS. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030589
American Medical Association (AMA)
Paz, Tiago Antunes& Santos, Vânia A. F. F. M.& Inácio, Marielle Cascaes& Pina, Edieidia Souza& Pereira, Ana Maria S.& Furlan, Maysa. Production of the Quinone-Methide Triterpene Maytenin by In Vitro Adventitious Roots of Peritassa campestris (Cambess.) A.C.Sm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MSMS. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030589
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030589