High Insulin and Leptin Increase Resistin and Inflammatory Cytokine Production from Human Mononuclear Cells
Joint Authors
Tsiotra, Panayoula C.
Raptis, Sotirios A.
Dimitriadis, George
Boutati, Eleni
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Resistin and the proinflammatory cytokines, such as TNF-α, IL-6, and IL-1β, produced by adipocytes, and macrophages, are considered to be important modulators of chronic inflammation contributing to the development of obesity and atherosclerosis.
Human monocyte-enriched mononuclear cells, from ten healthy individuals, were exposed to high concentrations of insulin, leptin, and glucose (alone or in combination) for 24 hours in vitro.
Resistin, TNF-α, IL-6, and IL-1β production was examined and compared to that in untreated cells.
High insulin and leptin concentrations significantly upregulated resistin and the cytokines.
The subsequent addition of high glucose significantly upregulated resistin and TNF-α mRNA and protein secretion, while it did not have any effect on IL-6 or IL-1β production.
By comparison, exposure to dexamethasone reduced TNF-α, IL-6, and IL-1β production, while at this time point it increased resistin protein secretion.
These data suggest that the expression of resistin, TNF-α, IL-6, and IL-1β from human mononuclear cells, might be enhanced by the hyperinsulinemia and hyperleptinemia and possibly by the hyperglycemia in metabolic diseases as obesity, type 2 diabetes, and atherosclerosis.
Therefore, the above increased production may contribute to detrimental effects of their increased adipocyte-derived circulating levels on systemic inflammation, insulin sensitivity, and endothelial function of these patients.
American Psychological Association (APA)
Tsiotra, Panayoula C.& Boutati, Eleni& Dimitriadis, George& Raptis, Sotirios A.. 2012. High Insulin and Leptin Increase Resistin and Inflammatory Cytokine Production from Human Mononuclear Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030590
Modern Language Association (MLA)
Tsiotra, Panayoula C.…[et al.]. High Insulin and Leptin Increase Resistin and Inflammatory Cytokine Production from Human Mononuclear Cells. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030590
American Medical Association (AMA)
Tsiotra, Panayoula C.& Boutati, Eleni& Dimitriadis, George& Raptis, Sotirios A.. High Insulin and Leptin Increase Resistin and Inflammatory Cytokine Production from Human Mononuclear Cells. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030590
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030590