Does Cryopreservation of Ovarian Tissue Affect the Distribution and Function of Germinal Vesicle Oocytes Mitochondria?
Joint Authors
Salehnia, Mojdeh
Töhönen, Virpi
Zavareh, Saeed
Inzunza, Jose
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this study was to evaluate mitochondrial alteration and ATP content of germinal vesicle (GV) oocytes isolated from fresh and vitrified ovaries.
After superovulation, the ovaries from adult mice were collected and divided into control and vitrified groups.
GV oocytes were isolated mechanically from each group.
Half were cultured for 24 hours and their maturation was assessed.
Metaphase II oocytes were collected and submitted to in vitro fertilization and their fertilization rates and development to the blastocyst stage were evaluated.
In the remaining GV oocytes, ATP levels were quantified, and mitochondrial distribution, mitochondrial membrane potential, and intracellular free calcium were detected with rhodamine 123, JC-1 and Flou-4 AM staining, using laser-scanning confocal microscopy.
Maturation and fertilization rates of GV oocytes and the developmental rates of subsequent embryos were significantly lower in vitrified samples (P<0.05).
The ATP content and Ca2+ levels differed significantly in fresh and vitrified GV oocytes (P<0.05).
Most mitochondria were seen as large and homogenous aggregates (66.6%) in fresh GV oocytes compared to vitrified oocytes (50%).
No significant differences in mitochondrial membrane potential were found between the groups.
The lower maturation and fertilization rates of GV oocytes from vitrified ovaries may be due to changes in their mitochondrial function and distribution.
American Psychological Association (APA)
Salehnia, Mojdeh& Töhönen, Virpi& Zavareh, Saeed& Inzunza, Jose. 2013. Does Cryopreservation of Ovarian Tissue Affect the Distribution and Function of Germinal Vesicle Oocytes Mitochondria?. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030592
Modern Language Association (MLA)
Salehnia, Mojdeh…[et al.]. Does Cryopreservation of Ovarian Tissue Affect the Distribution and Function of Germinal Vesicle Oocytes Mitochondria?. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030592
American Medical Association (AMA)
Salehnia, Mojdeh& Töhönen, Virpi& Zavareh, Saeed& Inzunza, Jose. Does Cryopreservation of Ovarian Tissue Affect the Distribution and Function of Germinal Vesicle Oocytes Mitochondria?. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030592
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030592