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Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity
Joint Authors
Milano, Giuseppina
Roehrich, Marc-Estienne
Spicher, Albert
Vassalli, Giuseppe
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-31
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
High aldehyde dehydrogenase (ALDH) activity has been associated with stem and progenitor cells in various tissues.
Human cord blood and bone marrow ALDH-bright (ALDHbr) cells have displayed angiogenic activity in preclinical studies and have been shown to be safe in clinical trials in patients with ischemic cardiovascular disease.
The presence of ALDHbr cells in the heart has not been evaluated so far.
We have characterized ALDHbr cells isolated from mouse hearts.
One percent of nonmyocytic cells from neonatal and adult hearts were ALDHbr.
ALDHvery-br cells were more frequent in neonatal hearts than adult.
ALDHbr cells were more frequent in atria than ventricles.
Expression of ALDH1A1 isozyme transcripts was highest in ALDHvery-br cells, intermediate in ALDHbr cells, and lowest in ALDHdim cells.
ALDH1A2 expression was highest in ALDHvery-br cells, intermediate in ALDHdim cells, and lowest in ALDHbr cells.
ALDH1A3 and ALDH2 expression was detectable in ALDHvery-br and ALDHbr cells, unlike ALDHdim cells, albeit at lower levels compared with ALDH1A1 and ALDH1A2.
Freshly isolated ALDHbr cells were enriched for cells expressing stem cell antigen-1, CD34, CD90, CD44, and CD106.
ALDHbr cells, unlike ALDHdim cells, could be grown in culture for more than 40 passages.
They expressed sarcomeric α-actinin and could be differentiated along multiple mesenchymal lineages.
However, the proportion of ALDHbr cells declined with cell passage.
In conclusion, the cardiac-derived ALDHbr population is enriched for progenitor cells that exhibit mesenchymal progenitor-like characteristics and can be expanded in culture.
The regenerative potential of cardiac-derived ALDHbr cells remains to be evaluated.
American Psychological Association (APA)
Roehrich, Marc-Estienne& Spicher, Albert& Milano, Giuseppina& Vassalli, Giuseppe. 2013. Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity. BioMed Research International،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1030605
Modern Language Association (MLA)
Roehrich, Marc-Estienne…[et al.]. Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity. BioMed Research International No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-1030605
American Medical Association (AMA)
Roehrich, Marc-Estienne& Spicher, Albert& Milano, Giuseppina& Vassalli, Giuseppe. Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1030605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030605