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Affinity Labeling of Membrane Receptors Using Tissue-Penetrating Radiations
Joint Authors
Boja, John
Ho, Beng
Kuhar, Michael J.
Wong, Dean F.
Wong, Franklin C. L.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Photoaffinity labeling, a useful in vivo biochemical tool, is limited when applied in vivo because of the poor tissue penetration by ultraviolet (UV) photons.
This study investigates affinity labeling using tissue-penetrating radiation to overcome the tissue attenuation and irreversibly label membrane receptor proteins.
Using X-ray (115 kVp) at low doses (<50 cGy or Rad), specific and irreversible binding was found on striatal dopamine transporters with 3 photoaffinity ligands for dopamine transporters, to different extents.
Upon X-ray exposure (115 kVp), RTI-38 and RTI-78 ligands showed irreversible and specific binding to the dopamine transporter similar to those seen with UV exposure under other conditions.
Similarly, gamma rays at higher energy (662 keV) also affect irreversible binding of photoreactive ligands to peripheral benzodiazepine receptors (by PK14105) and to the dopamine (D2) membrane receptors (by azidoclebopride), respectively.
This study reports that X-ray and gamma rays induced affinity labeling of membrane receptors in a manner similar to UV with photoreactive ligands of the dopamine transporter, D2 dopamine receptor (D2R), and peripheral benzodiazepine receptor (PBDZR).
It may provide specific noninvasive irreversible block or stimulation of a receptor using tissue-penetrating radiation targeting selected anatomic sites.
American Psychological Association (APA)
Wong, Franklin C. L.& Boja, John& Ho, Beng& Kuhar, Michael J.& Wong, Dean F.. 2013. Affinity Labeling of Membrane Receptors Using Tissue-Penetrating Radiations. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030606
Modern Language Association (MLA)
Wong, Franklin C. L.…[et al.]. Affinity Labeling of Membrane Receptors Using Tissue-Penetrating Radiations. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030606
American Medical Association (AMA)
Wong, Franklin C. L.& Boja, John& Ho, Beng& Kuhar, Michael J.& Wong, Dean F.. Affinity Labeling of Membrane Receptors Using Tissue-Penetrating Radiations. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030606
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030606