The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing

Abstract EN

Objective.

To evaluate the benefits of conditioned medium of Adipose-derived stem cells (ADSC-CM) on wound healing after fractional carbon dioxide laser resurfacing (FxCR) on human skin.

Materials and Methods.

Nineteen subjects were treated with FxCR on the bilateral inner arms.

ADSC-CM was applied on FxCR site of one randomly selected arm.

Transepidermal water loss (TEWL), skin color, and gross-elasticity of FxCR site on both arms were measured.

Skin samples were taken by biopsy from three subjects 3 weeks after treatment for histopathological manifestations and mRNA expressions of procollagen types I and III, elastin genes were noted.

Results.

The index of erythema, melanin, and TEWL of the ADSC-CM-treated skin were significantly lower than those of the control side.

The mRNA expression of type III procollagen in ADSC-CM-treated group at 3 weeks posttreatment was 2.6 times of that of the control group.

Conclusion.

Application of allograft ADSC-CM is an effective method for enhancing wound healing after FxCR, by reducing transient adverse effects such as erythema, hyperpigmentation, and increased TEWL.