Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
Joint Authors
Shimizu, Tatsuya
Fu, Xian-ming
Lee, Jong-Kook
Miwa, Keiko
Takagishi, Yoshiko
Hirabayashi, Masumi
Usui, Akihiko
Kodama, Itsuo
Ueda, Yuichi
Watabe, Kazuhiko
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of myocardial tissue engineering is to repair or regenerate damaged myocardium with engineered cardiac tissue.
However, this strategy has been hampered by lack of functional integration of grafts with native myocardium.
Autonomic innervation may be crucial for grafts to function properly with host myocardium.
In this study, we explored the feasibility of in vivo induction of autonomic innervation to engineered myocardial tissue using genetic modulation by adenovirus encoding glial cell line derived neurotrophic factor (GDNF).
GFP-transgene (control group) or GDNF overexpressing (GDNF group) engineered cardiomyocyte sheets were transplanted on cryoinjured hearts in rats.
Nerve fibers in the grafts were examined by immunohistochemistry at 1, 2, and 4 weeks postoperatively.
Growth associated protein-43 positive growing nerves and tyrosine hydroxylase positive sympathetic nerves were first detected in the grafts at 2 weeks postoperatively in control group and 1 week in GDNF group.
The densities of growing nerve and sympathetic nerve in grafts were significantly increased in GDNF group.
No choline acetyltransferase immunopositive parasympathetic nerves were observed in grafts.
In conclusion, sympathetic innervation could be effectively induced into engrafted engineered cardiomyocyte sheets using GDNF.
American Psychological Association (APA)
Fu, Xian-ming& Lee, Jong-Kook& Miwa, Keiko& Shimizu, Tatsuya& Takagishi, Yoshiko& Hirabayashi, Masumi…[et al.]. 2013. Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030636
Modern Language Association (MLA)
Fu, Xian-ming…[et al.]. Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030636
American Medical Association (AMA)
Fu, Xian-ming& Lee, Jong-Kook& Miwa, Keiko& Shimizu, Tatsuya& Takagishi, Yoshiko& Hirabayashi, Masumi…[et al.]. Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030636
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030636