Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells
Joint Authors
Kim, Jin-Hoi
Gurunathan, Sangiliyandi
Han, Jae Woong
Eppakayala, Vasuki
Jeyaraj, Muniyandi
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Silver nanoparticles (AgNPs) have been used as an antimicrobial and disinfectant agents.
However, there is limited information about antitumor potential.
Therefore, this study focused on determining cytotoxic effects of AgNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death.
Herein, we developed a green method for synthesis of AgNPs using culture supernatant of Bacillus funiculus, and synthesized AgNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, particle size analyzer, and transmission electron microscopy (TEM).
The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress.
MDA-MB-231 breast cancer cells were treated with various concentrations of AgNPs (5 to 25 μg/mL) for 24 h.
We found that AgNPs inhibited the growth in a dose-dependent manner using MTT assay.
AgNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH), caspase-3, reactive oxygen species (ROS) generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation.
The present results showed that AgNPs might be a potential alternative agent for human breast cancer therapy.
American Psychological Association (APA)
Gurunathan, Sangiliyandi& Han, Jae Woong& Eppakayala, Vasuki& Jeyaraj, Muniyandi& Kim, Jin-Hoi. 2013. Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030638
Modern Language Association (MLA)
Gurunathan, Sangiliyandi…[et al.]. Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030638
American Medical Association (AMA)
Gurunathan, Sangiliyandi& Han, Jae Woong& Eppakayala, Vasuki& Jeyaraj, Muniyandi& Kim, Jin-Hoi. Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030638
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030638