Effect of Jatropha curcas Peptide Fractions on the Angiotensin I-Converting Enzyme Inhibitory Activity
Joint Authors
Peralta González, Fanny
Betancur-Ancona, David A.
Chel-Guerrero, Luis A.
Segura-Campos, Maira Rubi
Castellanos-Ruelas, Arturo F.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Hypertension is one of the most common worldwide diseases in humans.
Angiotensin I-converting enzyme (ACE) plays an important role in regulating blood pressure and hypertension.
An evaluation was done on the effect of Alcalase hydrolysis of defatted Jatropha curcas kernel meal on ACE inhibitory activity in the resulting hydrolysate and its purified fractions.
Alcalase exhibited broad specificity and produced a protein hydrolysate with a 21.35% degree of hydrolysis and 34.87% ACE inhibition.
Ultrafiltration of the hydrolysate produced peptide fractions with increased biological activity (24.46–61.41%).
Hydrophobic residues contributed substantially to the peptides’ inhibitory potency.
The 5–10 and <1 kDa fractions were selected for further fractionation by gel filtration chromatography.
ACE inhibitory activity (%) ranged from 22.66 to 45.96% with the 5–10 kDa ultrafiltered fraction and from 36.91 to 55.83% with the <1 kDa ultrafiltered fraction.
The highest ACE inhibitory activity was observed in F2 (IC50=6.7 μg/mL) from the 5–10 kDa fraction and F1 (IC50=4.78 μg/mL) from the <1 kDa fraction.
ACE inhibitory fractions from Jatropha kernel have potential applications in alternative hypertension therapies, adding a new application for the Jatropha plant protein fraction and improving the financial viability and sustainability of a Jatropha-based biodiesel industry.
American Psychological Association (APA)
Segura-Campos, Maira Rubi& Peralta González, Fanny& Castellanos-Ruelas, Arturo F.& Chel-Guerrero, Luis A.& Betancur-Ancona, David A.. 2013. Effect of Jatropha curcas Peptide Fractions on the Angiotensin I-Converting Enzyme Inhibitory Activity. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030646
Modern Language Association (MLA)
Segura-Campos, Maira Rubi…[et al.]. Effect of Jatropha curcas Peptide Fractions on the Angiotensin I-Converting Enzyme Inhibitory Activity. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030646
American Medical Association (AMA)
Segura-Campos, Maira Rubi& Peralta González, Fanny& Castellanos-Ruelas, Arturo F.& Chel-Guerrero, Luis A.& Betancur-Ancona, David A.. Effect of Jatropha curcas Peptide Fractions on the Angiotensin I-Converting Enzyme Inhibitory Activity. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030646
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030646