Development of a Generic Microfluidic Device for Simultaneous Detection of Antibodies and Nucleic Acids in Oral Fluids

Abstract EN

A prototype dual-path microfluidic device (Rheonix CARD) capable of performing simultaneously screening (antigen or antibody) and confirmatory (nucleic acid) detection of pathogens is described.

The device fully integrates sample processing, antigen or antibody detection, and nucleic acid amplification and detection, demonstrating rapid and inexpensive “sample-to-result” diagnosis with performance comparable to benchtop analysis.

For the chip design, a modular approach was followed allowing the optimization of individual steps in the sample processing process.

This modular design provides great versatility accommodating different disease targets independently of the production method.

In the detection module, a lateral flow (LF) protocol utilizing upconverting phosphor (UCP) reporters was employed.

The nucleic acid (NA) module incorporates a generic microtube containing dry reagents.

Lateral flow strips and PCR primers determine the target or disease that is diagnosed.

Diagnosis of HIV infection was used as a model to investigate the simultaneous detection of both human antibodies against the virus and viral RNA.

The serological result is available in less than 30 min, and the confirmation by RNA amplification takes another 60 min.

This approach combines a core serological portable diagnostic with a nucleic acid-based confirmatory test.