Transcriptomic Profiling of the Four Adenosine Receptors in Human Leukocytes of Heart Failure Patients
Joint Authors
Cabiati, Manuela
Verde, Alessandro
Morales, Maria-Aurora
Sabatino, L.
Del Ry, S.
Caruso, Raffaele
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In this study the transcriptomic profiling of adenosine receptors (ARs) in human leukocytes of heart failure (HF) patients as a function of clinical severity, assessing the possible changes with respect to healthy subjects (C), was evaluated.
Total RNA was extracted from leukocytes of C (n=8) and of HF patients (NYHA I-II n=9; NYHA III-IV n=14) with a PAXgene Blood RNA Kit.
An increase as a function of clinical severity was observed in each AR (A1R: C=0.02±0.009, NYHA I-II=0.21±0.09, NYHA III-IV=3.6±1.3, P=0.03 C versus NYHA III-IV, P=0.02 NYHA I-II versus NYHA III-IV; A2aR: C=0.2±0.05, NYHA I-II=0.19±0.04, NYHA III-IV=1.32±0.33, P=0.005 C versus NYHA III-IV, P=0.003 NYHA I-II versus NYHA III-IV; A2bR: C=1.78±0.36, NYHA I-II=1.35±0.29, NYHA III-IV=4.07±1.21, P=0.03: NYHA I-II versus NYHA III-IV; A3R: C=0.76±0.21, NYHA I-II=0.94±0.19, NYHA III-IV=3.14±0.77, P=0.01 C versus NYHA III-IV and NYHA I-II versus NYHA III-IV, resp.).
The mRNA expression of the ectonucleoside triphosphate diphosphohydrolase (CD39) and the ecto-5′-nucleotidase (CD73) were also evaluated.
They resulted up-regulated.
These findings show that components of adenosine metabolism and signalling are altered to promote adenosine production and signalling in HF patients.
Thus, HF may benefit from adenosine-based drug therapy after confirmation by clinical trials.
American Psychological Association (APA)
Cabiati, Manuela& Caruso, Raffaele& Verde, Alessandro& Sabatino, L.& Morales, Maria-Aurora& Del Ry, S.. 2013. Transcriptomic Profiling of the Four Adenosine Receptors in Human Leukocytes of Heart Failure Patients. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030669
Modern Language Association (MLA)
Cabiati, Manuela…[et al.]. Transcriptomic Profiling of the Four Adenosine Receptors in Human Leukocytes of Heart Failure Patients. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1030669
American Medical Association (AMA)
Cabiati, Manuela& Caruso, Raffaele& Verde, Alessandro& Sabatino, L.& Morales, Maria-Aurora& Del Ry, S.. Transcriptomic Profiling of the Four Adenosine Receptors in Human Leukocytes of Heart Failure Patients. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1030669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030669