Neurogenesis and Increase in Differentiated Neural Cell Survival via Phosphorylation of Akt1 after Fluoxetine Treatment of Stem Cells
Joint Authors
Shoae-Hassani, Alireza
Kheradmand, Danial
Keyhanvar, Peyman
Darbandi-Azar, Amir
Rahmani, Anahita
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Fluoxetine (FLX) is a selective serotonin reuptake inhibitor (SSRI).
Its action is possibly through an increase in neural cell survival.
The mechanism of improved survival rate of neurons by FLX may relate to the overexpression of some kinases such as Akt protein.
Akt1 (a serine/threonine kinase) plays a key role in the modulation of cell proliferation and survival.
Our study evaluated the effects of FLX on mesenchymal stem cell (MSC) fate and Akt1 phosphorylation levels in MSCs.
Evaluation tests included reverse transcriptase polymerase chain reaction, western blot, and immunocytochemistry assays.
Nestin, MAP-2, and β-tubulin were detected after neurogenesis as neural markers.
Ten μM of FLX upregulated phosphorylation of Akt1 protein in induced hEnSC significantly.
Also FLX did increase viability of these MSCs.
Continuous FLX treatment after neurogenesis elevated the survival rate of differentiated neural cells probably by enhanced induction of Akt1 phosphorylation.
This study addresses a novel role of FLX in neurogenesis and differentiated neural cell survival that may contribute to explaining the therapeutic action of fluoxetine in regenerative pharmacology.
American Psychological Association (APA)
Rahmani, Anahita& Kheradmand, Danial& Keyhanvar, Peyman& Shoae-Hassani, Alireza& Darbandi-Azar, Amir. 2013. Neurogenesis and Increase in Differentiated Neural Cell Survival via Phosphorylation of Akt1 after Fluoxetine Treatment of Stem Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030684
Modern Language Association (MLA)
Rahmani, Anahita…[et al.]. Neurogenesis and Increase in Differentiated Neural Cell Survival via Phosphorylation of Akt1 after Fluoxetine Treatment of Stem Cells. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030684
American Medical Association (AMA)
Rahmani, Anahita& Kheradmand, Danial& Keyhanvar, Peyman& Shoae-Hassani, Alireza& Darbandi-Azar, Amir. Neurogenesis and Increase in Differentiated Neural Cell Survival via Phosphorylation of Akt1 after Fluoxetine Treatment of Stem Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030684
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030684