Biochemical Characterization and Pharmacological Properties of New Basic PLA2 BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom
Joint Authors
Gomes Heleno, Mauricio Aurelio
Ponce Soto, L. A.
Baldasso, P. A.
Marangoni, Sérgio
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
BrTX-I, a PLA2, was purified from Bothrops roedingeri venom after only one chromatographic step using reverse-phase HPLC on μ-Bondapak C-18 column.
A molecular mass of 14358.69 Da was determined by MALDI-TOF mass spectrometry.
Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues.
The total amino acid sequence was obtained using SwissProt database and showed high amino acid sequence identity with other PLA2 from snake venom.
The amino acid composition showed that BrTX-I has a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical of a basic PLA2.
BrTX-I presented PLA2 activity and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0, 35–45°C, and required Ca2+.
In vitro, the whole venom and BrTX-I caused a neuromuscular blockade in biventer cervicis preparations in a similar way to other Bothrops species.
BrTX-I induced myonecrosis and oedema-forming activity analyzed through injection of the purified BrTX-I in mice.
Since BrTX-I exerts a strong proinflammatory effect, the enzymatic phospholipid hydrolysis might be relevant for these phenomena; incrementing levels of IL-1, IL-6, and TNFα were observed at 15 min, 30 min, one, two, and six hours postinjection, respectively.
American Psychological Association (APA)
Gomes Heleno, Mauricio Aurelio& Baldasso, P. A.& Ponce Soto, L. A.& Marangoni, Sérgio. 2012. Biochemical Characterization and Pharmacological Properties of New Basic PLA2 BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030696
Modern Language Association (MLA)
Gomes Heleno, Mauricio Aurelio…[et al.]. Biochemical Characterization and Pharmacological Properties of New Basic PLA2 BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1030696
American Medical Association (AMA)
Gomes Heleno, Mauricio Aurelio& Baldasso, P. A.& Ponce Soto, L. A.& Marangoni, Sérgio. Biochemical Characterization and Pharmacological Properties of New Basic PLA2 BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030696
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030696